人呼吸道中乙酰胆碱的降解：丁酰胆碱酯酶的作用
Degradation of acetylcholine in human airways: role of butyrylcholinesterase.
作者信息
Norel X, Angrisani M, Labat C, Gorenne I, Dulmet E, Rossi F, Brink C
机构信息
CNRS URA 1159, Centre Chirurgical Marie-Lannelongue, France.
出版信息
Br J Pharmacol. 1993 Apr;108(4):914-9. doi: 10.1111/j.1476-5381.1993.tb13486.x.
Abstract
- Neostigmine and BW284C51 induced concentration-dependent contractions in human isolated bronchial preparations whereas tetraisopropylpyrophosphoramide (iso-OMPA) was inactive on airway resting tone. 2. Neostigmine (0.1 microM) or iso-OMPA (100 microM) increased acetylcholine sensitivity in human isolated bronchial preparations but did not alter methacholine or carbachol concentration-effect curves. 3. In the presence of iso-OMPA (10 microM) the bronchial rings were more sensitive to neostigmine. The pD2 values were, control: 6.05 +/- 0.15 and treated: 6.91 +/- 0.14. 4. Neostigmine or iso-OMPA retarded the degradation of acetylcholine when this substrate was exogenously added to human isolated airways. A marked reduction of acetylcholine degradation was observed in the presence of both inhibitors. Exogenous butyrylcholine degradation was prevented by iso-OMPA (10 microM) but not by neostigmine (0.1 microM). 5. These results suggest the presence of butyrylcholinesterase activity in human bronchial muscle and this enzyme may co-regulate the degradation of acetylcholine in this tissue.
摘要
- 新斯的明和BW284C51在人离体支气管标本中诱导浓度依赖性收缩，而四异丙基焦磷酰胺（iso - OMPA）对气道静息张力无作用。2. 新斯的明（0.1微摩尔）或iso - OMPA（100微摩尔）增加人离体支气管标本对乙酰胆碱的敏感性，但不改变乙酰甲胆碱或卡巴胆碱的浓度 - 效应曲线。3. 在存在iso - OMPA（10微摩尔）的情况下，支气管环对新斯的明更敏感。pD2值分别为，对照：6.05±0.15，处理后：6.91±0.14。4. 当外源性添加乙酰胆碱底物至人离体气道时，新斯的明或iso - OMPA延缓其降解。在两种抑制剂同时存在时观察到乙酰胆碱降解明显减少。外源性丁酰胆碱的降解被iso - OMPA（10微摩尔）阻止，但不被新斯的明（0.1微摩尔）阻止。5. 这些结果提示人支气管肌中存在丁酰胆碱酯酶活性，且该酶可能共同调节该组织中乙酰胆碱的降解。
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