Roy Bidhan C, Santos Marina, Mallik Sanku, Campiglia Andres D
Department of Chemistry, North Dakota State University, Fargo 58105, USA.
J Org Chem. 2003 May 16;68(10):3999-4007. doi: 10.1021/jo026833k.
Sensitization of lanthanide ions is important for lanthanide ion-based assays and sensing. To the best of our knowledge, there are very few reports of lanthanide ion sensitization after it is incorporated into the liposome surface. This paper describes the syntheses of several saturated and polymerizable metal-chelating lipids based on chelidamic acid. The lipids are synthesized either from (S)-ornithine or racemic 2,3-diaminopropanoic acid. These lipids as well as polymerized liposomes incorporating these lipids sensitize lanthanide ions. Liposomes from the lipid 18-Eu(3+) provided a probe that relies not only on the emission wavelengths of Eu(3+) but also on a reproducible lifetime that can be used for protein identification.
镧系离子的敏化对于基于镧系离子的分析和传感很重要。据我们所知，关于镧系离子掺入脂质体表面后的敏化报道非常少。本文描述了几种基于乙二胺四乙酸的饱和及可聚合金属螯合脂质的合成。这些脂质由（S）-鸟氨酸或外消旋2,3-二氨基丙酸合成。这些脂质以及掺入这些脂质的聚合脂质体可使镧系离子敏化。脂质18-Eu(3+)形成的脂质体提供了一种探针，它不仅依赖于Eu(3+)的发射波长，还依赖于可用于蛋白质鉴定的可重现寿命。
