Santos Marina, Roy Bidhan C, Goicoechea Héctor, Campiglia Andres D, Mallik Sanku
Department of Chemistry, P.O. Box 25000, University of Central Florida, Orlando, Florida 32816-2366, USA.
J Am Chem Soc. 2004 Sep 1;126(34):10738-45. doi: 10.1021/ja048963b.
We present a promising approach to protein sensing based on Eu3+ ions incorporated into polymerized liposomes. The sensitization of Eu3+ is accomplished with 5-aminosalicylic acid, which provides energy transfer for a stable reference signal and a wide wavelength excitation range free from protein interference. The lipophilic character of polymerized liposomes provides the appropriate platform for protein interaction with the lanthanide ion. Quantitative analysis is based on the linear relationship between the luminescence signal of Eu3+ and protein concentration. Because no spectral shift of the lanthanide luminescence is observed upon protein interaction, qualitative analysis is based on the luminescence lifetime of polymerized liposomes. This parameter, which changes significantly upon protein-liposome interaction, follows a well-behaved single-exponential decay that might be useful for protein identification.
我们提出了一种基于掺入聚合脂质体中的铕离子的蛋白质传感方法。铕离子的敏化是通过5-氨基水杨酸实现的，它为稳定的参考信号提供能量转移，并提供一个不受蛋白质干扰的宽波长激发范围。聚合脂质体的亲脂特性为蛋白质与镧系离子的相互作用提供了合适的平台。定量分析基于铕离子的发光信号与蛋白质浓度之间的线性关系。由于在蛋白质相互作用时未观察到镧系元素发光的光谱位移，定性分析基于聚合脂质体的发光寿命。该参数在蛋白质-脂质体相互作用时会发生显著变化，遵循良好的单指数衰减，这可能有助于蛋白质识别。
