Kalousová Marta, Sulková Sylvie, Zima Tomás, Deppisch Reinhold, Beck Werner, Bednárová s Vladimíra, Fortová Magdaléna, Tesar Vladimír
Institute of Medical Biochemistry, 1st Faculty of Medicine, Charles University and University Hospital, Prague, Czech Republic.
Ren Fail. 2003 Mar;25(2):277-86. doi: 10.1081/jdi-120018728.
Advanced glycation end products (AGEs) and other carbonyl and oxidative stress compounds are supposed to play a critical role in the pathogenesis of several diseases and their complications, i.e., diabetes mellitus, diabetic retinopathy, atherosclerosis, and chronic renal failure. In the present investigation, we were interested in the relationship of AGEs in plasma to other prominent factors in the patients on chronic hemodialysis treatment-27 patients with diabetes mellitus, 35 patients without diabetes mellitus. AGE-group reactivity was estimated using a spectrofluorometric method (excitation 350 nm, emission 430 nm) and is expressed in arbitrary units (AU). We found significantly higher AGEs levels in diabetics than in non-diabetics on regular hemodialysis treatment both before (2.7 +/- 0.7 x 10(4) AU vs. 2.2 +/- 0.6 x 10(4) AU, p < 0.001) and after the dialysis session (2.3 +/- 0.5 x 10(4) AU vs. 1.8 +/- 0.7 x 10(4) AU, p < 0.005). AGEs were significantly reduced during hemodialysis in both groups of patients--by 15.4% in the diabetic go (p < 0.001) and by 17.3% in non-diabetics (p < 0.005). In the patients with diabetes mellitus, AGEs did not correlate with parameters of the glucose metabolism correction (blood glucose, HbA1c). We observed a significant correlation between AGEs and leptin (r = 0.48, p < 0.05) as well as the leptin/body fat ratio (r = 0.56, p < 0.05) only in hemodialyzed patients with diabetes mellitus. These findings suggest more detailed studies to identify the molecular links between carbonyl stress, i.e., advanced glycation end products, and leptin metabolism, sign of microinflammation and hypertension.
晚期糖基化终末产物（AGEs）以及其他羰基和氧化应激化合物被认为在多种疾病及其并发症（即糖尿病、糖尿病视网膜病变、动脉粥样硬化和慢性肾衰竭）的发病机制中起关键作用。在本研究中，我们关注慢性血液透析治疗患者血浆中AGEs与其他显著因素之间的关系——27例糖尿病患者和35例非糖尿病患者。采用荧光分光光度法（激发波长350nm，发射波长430nm）估算AGE组反应性，并以任意单位（AU）表示。我们发现，在常规血液透析治疗中，糖尿病患者透析前（2.7±0.7×10⁴AU对2.2±0.6×10⁴AU，p<0.001）和透析后（2.3±0.5×10⁴AU对1.8±0.7×10⁴AU，p<0.005）的AGEs水平均显著高于非糖尿病患者。两组患者在血液透析过程中AGEs均显著降低——糖尿病组降低15.4%（p<0.001），非糖尿病组降低17.3%（p<0.005）。在糖尿病患者中，AGEs与血糖代谢校正参数（血糖、糖化血红蛋白）无关。仅在接受血液透析的糖尿病患者中，我们观察到AGEs与瘦素之间存在显著相关性（r=0.48，p<0.05），以及瘦素/体脂比值之间存在显著相关性（r=0.56，p<0.05）。这些发现表明需要进行更详细的研究，以确定羰基应激（即晚期糖基化终末产物）与瘦素代谢、微炎症迹象和高血压之间的分子联系。
