Cobb George P, Bargar Tim A, Pepper Chris B, Norman Don M, Houlis Pattie D, Anderson Todd A
The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79416, USA.
Ecotoxicology. 2003 Feb-Aug;12(1-4):31-45. doi: 10.1023/a:1022532711353.
David Peakall and co-workers pioneered innovative approaches that utilized extra-embryonic membranes to assess accumulation of organochlorine pesticides in eggs. This technique provided the foundation for an entire line of research to improve non-lethal methods for assessing contaminant exposure in oviparous wildlife. Currently, analysis of chorioallantoic membranes (CAMs) provides predictable estimates of chlorinated contaminant presence in eggs and in maternal tissues. Field studies have been conducted with herons, stilts, alligators, crocodiles, and sea turtles. Controlled dose-response studies have been completed in chickens. The following manuscript presents the foundations for the CAM approach and a review of research findings involving this technique.
大卫·皮卡尔及其同事开创了创新方法，利用胚外膜来评估有机氯农药在卵中的积累情况。这项技术为一整套研究奠定了基础，旨在改进评估卵生野生动物污染物暴露的非致死方法。目前，对尿囊绒膜（CAMs）的分析能够对卵和母体组织中氯化污染物的存在情况提供可预测的估计。已经对苍鹭、长脚鹬、短吻鳄、鳄鱼和海龟进行了实地研究。在鸡身上完成了对照剂量反应研究。以下手稿介绍了CAM方法的基础以及对涉及该技术的研究结果的综述。
