Cobb G P, Norman D M, Kendall R J
The Department of Environmental Toxicology and The Institute of Wildlife and Environmental Toxicology (TIWET), Clemson University, Pendleton, South Carolina 29670, USA.
Environ Pollut. 1994;83(3):299-309. doi: 10.1016/0269-7491(94)90151-1.
A nonlethal method is discussed for the evaluation of contaminant concentrations in whole eggs. Concentrations of pentachlorobenzene, hexachlorobenzene, DDE, and the PCB congeners, BZ-60, BZ-118, BZ-138, BZ-180, and BZ-170 were quantified in tissue samples from great blue herons (Ardea herodias). All tissues within whole eggs from two colonies were homogenized together and analysed for these chlorinated contaminants. Contents and chorio-allantoic membranes (CAMs) of additional whole eggs were separated and analysed. Contaminant distributions were determined for the CAM and contents of whole eggs from the same colonies. CAM tissues remaining in hatched eggs were also analysed for comparative purposes. Utilizing the distributions derived for contaminants between CAM and egg contents and the chemical concentrations determined in CAMs from hatched eggs, contaminant burdens in whole eggs were calculated. This process produced concentration estimates that described actual, whole egg burdens of chlorinated contaminants within a factor of 2 (1.03-3.7). Contaminant burdens in eggs from the two colonies were also statistically different for DDE and total PCB concentrations.
本文讨论了一种用于评估全蛋中污染物浓度的非致死方法。对大蓝鹭（Ardea herodias）组织样本中的五氯苯、六氯苯、滴滴涕以及多氯联苯同系物BZ - 60、BZ - 118、BZ - 138、BZ - 180和BZ - 170的浓度进行了定量分析。来自两个鸟群的全蛋中的所有组织被混合在一起进行匀浆，并对这些氯化污染物进行分析。另外一些全蛋的内容物和尿囊绒膜（CAMs）被分离并进行分析。确定了来自相同鸟群的全蛋中CAM和内容物的污染物分布情况。为了进行比较，还对孵化后蛋中剩余的CAM组织进行了分析。利用CAM和蛋内容物之间污染物的分布情况以及孵化后蛋中CAMs所测定的化学浓度，计算了全蛋中的污染物负荷。这一过程得出的浓度估计值与氯化污染物在全蛋中的实际负荷相差在2倍以内（1.03 - 3.7）。两个鸟群的蛋中滴滴涕和总多氯联苯浓度的污染物负荷在统计学上也存在差异。
