Kuzyk Zou Zou A, Burgess Neil M, Stow Jason P, Fox Glen A
Environmental Sciences Group, Royal Military College of Canada, Kingston, ON, Canada K7K 7B4.
Ecotoxicology. 2003 Feb-Aug;12(1-4):183-97. doi: 10.1023/a:1022550709962.
Black guillemots (Cepphus grylle) in Saglek Bay, Labrador have elevated polychlorinated biphenyl (PCB) concentrations due to marine sediment contamination around a former military site. We measured liver biomarkers and sigmaPCB concentrations in 31 nestlings from three PCB-exposure groups: Reference group (range: 15-46 ng/g liver, wet wt.), moderately exposed Islands group (24-150 ng/g), and highly exposed Beach group (170-6200 ng/g). Biomarker responses were dose-dependent and in some cases sex-dependent. Livers of female Beach nestlings were enlarged 36% relative to Reference females. In both sexes, Beach nestlings had liver ethoxyresorufin-O-deethylase (EROD) activities elevated 79% and liver retinol concentrations reduced 47%. Retinyl palmitate concentrations were reduced 50% but only among female nestlings. Island nestlings also exhibited EROD induction (57%) and reductions in retinol and retinyl palmitate concentrations (28 and 58%, respectively). Liver lipid content increased with sigmaPCBs in both sexes, and correlated with liver mass in males. Malic enzyme activity and porphyrin concentrations showed little association with sigmaPCBs. Although similar associations between liver biomarkers and organochlorine exposure in fish-eating birds are well documented, typically exposures involve multiple contaminants and there is uncertainty about specific PCB effects. Our findings indicate that liver biomarkers respond to relatively low PCB exposures (approximately 73 ng/g liver) in guillemots.
由于一个 former military site 周围的海洋沉积物受到污染，拉布拉多省萨格勒克湾的黑海鸠（Cepphus grylle）体内的多氯联苯（PCB）浓度升高。我们测量了来自三个 PCB 暴露组的 31 只雏鸟的肝脏生物标志物和总 PCB 浓度：对照组（范围：15 - 46 ng/g 肝脏，湿重）、中度暴露的岛屿组（24 - 150 ng/g）和高度暴露的海滩组（170 - 6200 ng/g）。生物标志物反应呈剂量依赖性，在某些情况下还存在性别依赖性。与对照组雌性相比，海滩组雌性雏鸟的肝脏增大了 36%。在两性中，海滩组雏鸟的肝脏乙氧基异吩唑酮 - O - 脱乙基酶（EROD）活性升高了 79%，肝脏视黄醇浓度降低了 47%。仅在雌性雏鸟中，棕榈酸视黄酯浓度降低了 50%。岛屿组雏鸟也表现出 EROD 诱导（57%）以及视黄醇和棕榈酸视黄酯浓度降低（分别为 28%和 58%）。两性的肝脏脂质含量均随总 PCB 浓度增加，且在雄性中与肝脏质量相关。苹果酸酶活性和卟啉浓度与总 PCB 浓度几乎没有关联。虽然在以鱼为食的鸟类中，肝脏生物标志物与有机氯暴露之间的类似关联已有充分记录，但通常暴露涉及多种污染物，并且特定 PCB 影响存在不确定性。我们的研究结果表明，肝脏生物标志物对黑海鸠中相对较低的 PCB 暴露（约 73 ng/g 肝脏）有反应。
