Kennedy Sean W, Fox Glen A, Jones Stephanie P, Trudeau Suzanne F
Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottawa, Ontario, Canada K1A 0H3.
Ecotoxicology. 2003 Feb-Aug;12(1-4):153-61. doi: 10.1023/a:1022594424983.
Liver concentrations of polychlorinated biphenyls (PCBs) and chicken embryo hepatocyte (CEH) bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (TCDD-EQs) were measured in livers of adult herring gulls (Larus argentatus) collected from several locations on the Great Lakes and two reference sites. Total PCB concentrations (sum of 42 congeners) and TCDD-EQ concentrations were compared with hepatic ethoxyresorufin-O-deethylase (EROD) activity, methoxyresorufin-O-deethylase (MROD) activity and immunodetectable cytochrome P4501A (CYP1A) protein concentration. EROD and MROD activity were not significantly correlated with total PCB concentration or TCDD-EQ concentration in liver tissue. CYP1A protein concentration was significantly correlated with total PCB concentration, but the linear relationship had little predictive power. We conclude that EROD is not a useful biomarker of PCB exposure in the adult herring gull.
在从五大湖的几个地点以及两个参考地点采集的成年银鸥（Larus argentatus）肝脏中，测量了多氯联苯（PCBs）的肝脏浓度以及鸡胚肝细胞（CEH）生物测定得出的2,3,7,8 - 四氯二苯并 - p - 二噁英当量（TCDD - EQs）。将总多氯联苯浓度（42种同系物的总和）和TCDD - EQ浓度与肝脏乙氧基 - 间苯二酚 - O - 脱乙基酶（EROD）活性、甲氧基 - 间苯二酚 - O - 脱乙基酶（MROD）活性以及免疫检测到的细胞色素P4501A（CYP1A）蛋白浓度进行了比较。EROD和MROD活性与肝脏组织中的总多氯联苯浓度或TCDD - EQ浓度无显著相关性。CYP1A蛋白浓度与总多氯联苯浓度显著相关，但线性关系的预测能力较弱。我们得出结论，EROD不是成年银鸥中多氯联苯暴露的有用生物标志物。
