Rubinstein L J, Conley F K, Herman M M
Acta Neuropathol. 1976 Apr 26;34(4):277-91. doi: 10.1007/BF00696558.
Explants from seven nerve sheath tumors (four cranial and three spinal) induced in rats by transplacental ethylnitrosourea were grown on collagen-coated coverslips and in organ culture systems, using sponge foam matrices and Millipore filter platforms. Their sequential morphological features in vitro were compared to those of a human acoustic Schwannoma maintained in similar culture systems. The original experimental tumors were either undifferentiated or poorly malignant Schwannomas. In cultures on collagen-coated coverslips the explants demonstrated cellular features that were considerably more anaplastic than those of the human acoustic Schwannoma. On the other hand, in organ culture systems, in which viable cultures were maintained up to 82 days, many of the experimental tumor explants exhibited progressive differentiaiton, with nuclear palisading, increasing whorl formation and abundant reticulin fibers, and their pattern of histological organization came therefore closely to resemble that of the cultured acoustic Schwannoma. Unlike the latter, however, the cultured experimental tumors infiltrated the sponge foam matrices, a feature that mimicked the invasive character of the original tumors in vivo. Included normal ganglion cells remained demonstrable in the cultures up to 69 in vitro.
将经胎盘给予乙基亚硝脲诱导产生的七例大鼠神经鞘瘤（四例颅神经鞘瘤和三例脊神经鞘瘤）的外植体，接种于涂有胶原的盖玻片上，并置于使用海绵泡沫基质和微孔滤膜平台的器官培养系统中培养。将其体外的连续形态学特征与在类似培养系统中培养的人类听神经鞘瘤的特征进行比较。最初的实验性肿瘤为未分化或低恶性的神经鞘瘤。在涂有胶原的盖玻片上培养时，外植体显示出的细胞特征比人类听神经鞘瘤的细胞特征更具间变。另一方面，在器官培养系统中，活培养物可维持长达82天，许多实验性肿瘤外植体表现出进行性分化，出现核栅栏状排列、漩涡形成增加和丰富的网状纤维，因此其组织学组织结构模式与培养的听神经鞘瘤非常相似。然而，与后者不同的是，培养的实验性肿瘤浸润海绵泡沫基质，这一特征模拟了原始肿瘤在体内的侵袭性。在体外培养长达69天时，培养物中仍可检测到正常神经节细胞。
