Horten B C, Montague S R
Acta Neuropathol. 1976 May 18;35(1):13-25. doi: 10.1007/BF00688940.
Explants of a human sacral chordoma were successfully maintained on collagen-coated coverslips, gelfoam sponge matrices, and Millipore filter platforms for up to 30 days. Tumor cells cultured on collagen-coated coverslips became increasingly vacuolated whereas those maintained in organ culture were entirely free of vacuoles after 22 days in vitro. A single basic tumor cell, small and polygonal with a large central spherical nucleus and abundant endoplasmic reticulum and Golgi apparatus, was recognized. Vacuoles were formed as the result of the progressive expansion of smooth endoplasmic reticulum. Coalescence of these vacuoles produced the physaliferous cell of light microscopy.
人骶骨脊索瘤外植体在胶原包被的盖玻片、明胶海绵基质和微孔滤膜平台上成功维持了长达30天。在胶原包被的盖玻片上培养的肿瘤细胞空泡化程度越来越高，而在器官培养中维持的肿瘤细胞在体外培养22天后完全没有空泡。识别出一种单一的基础肿瘤细胞，其小而呈多边形，有一个大的中央球形细胞核以及丰富的内质网和高尔基体。空泡是由滑面内质网的逐渐扩张形成的。这些空泡的融合产生了光镜下的泡沫细胞。
