Morré D James, Morré Dorothy M
Department of Medicinal Chemistry and Molecular Pharmacology, Hansen Life Sciences Research Building, Purdue University, 201 S. University Street, West Lafayette, IN 47907-2064, USA.
J Photochem Photobiol B. 2003 Apr;70(1):7-12. doi: 10.1016/s1011-1344(03)00023-x.
NADH oxidases of the external plasma membrane surface (ECTO-NOX proteins) are characterized by oscillations in activity with a regular period length of 24 min. Explants of mouse skin exhibit the oscillatory activity as estimated from the decrease in A(340) suggesting that individual ECTO-NOX molecules must somehow be induced to function synchronously. Transfer of explants of mouse skin from darkness to blue light (495 nm, 2 min, 50 micromol m(-1) s(-1)) resulted in initiation of a new activity maximum (entrainment) with a midpoint 36 min after light exposure followed by maxima every 24 min thereafter. Addition of melatonin resulted in a new maximum 24 min after melatonin addition. The findings suggest that the ECTO-NOX proteins play a central role in the entrainment of the biological clock both by light and by melatonin.
外质膜表面的NADH氧化酶（ECTO-NOX蛋白）的特征是其活性以24分钟的固定周期长度振荡。从小鼠皮肤外植体中A(340)的下降估计其具有振荡活性，这表明单个ECTO-NOX分子必须以某种方式被诱导同步发挥作用。将小鼠皮肤外植体从黑暗转移到蓝光（495纳米，2分钟，50微摩尔·米⁻²·秒⁻¹）中，会在光照后36分钟出现一个新的活性最大值（同步化），随后每24分钟出现一次最大值。添加褪黑素后，在添加褪黑素24分钟后会出现一个新的最大值。这些发现表明，ECTO-NOX蛋白在生物钟受光和褪黑素同步化过程中发挥着核心作用。
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