四膜虫和人血沉棕黄层细胞表面的光响应性及周期性NADH氧化酶活性。

A light-responsive and periodic NADH oxidase activity of the cell surface of Tetrahymena and of human buffy coat cells.

作者信息

Peter A D, Morré D J, Morré D M

机构信息

Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-1333, USA.

出版信息

Antioxid Redox Signal. 2000 Summer;2(2):289-300. doi: 10.1089/ars.2000.2.2-289.

DOI:10.1089/ars.2000.2.2-289

PMID:11229533

Abstract

Oxidation of external NADH (NADH is an impermeant substrate) by cells of Tetrahymena pyriformis oscillated with a period of 24-26 min. The period length in darkness (25.6 min) appeared to be slightly longer than the period in light (approximately 24 min). When Tetrahymena were placed in darkness for 30-50 min and then returned to light, a new maximum in the rate of NADH oxidation was observed 36-38 min (13 + 24) min after the beginning of the light treatment. The cell-surface NADH oxidase of human buffy coats (a mixture of white cells and platelets) also was periodic and light responsive.

摘要

梨形四膜虫细胞对外源NADH（NADH是一种不能透过细胞膜的底物）的氧化以24 - 26分钟的周期振荡。在黑暗中的周期长度（25.6分钟）似乎比在光照下的周期（约24分钟）略长。当梨形四膜虫在黑暗中放置30 - 50分钟后再回到光照下，在光照处理开始后36 - 38分钟（13 + 24分钟）观察到NADH氧化速率出现一个新的最大值。人血沉棕黄层（白细胞和血小板的混合物）的细胞表面NADH氧化酶也是周期性的且对光有反应。