Kato Satomi, Fogarty Elizabeth, Bowman Dwight
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaxa, New York 14853, USA.
Int J Environ Health Res. 2003 Jun;13(2):169-79. doi: 10.1080/0960312031000098071.
The viability of Cryptosporidium parvum oocysts and Ascaris suum eggs inoculated into aerobic and anaerobic digesters was measured. The digesters were maintained at 37 degrees C, 47 degrees C, and 55 degrees C, with 10-day detention times. Eggs and oocysts were added to each digester in a single spike or in chambers placed in the digesters for varying periods. Oocysts were inactivated very rapidly in all systems as determined by a dye permeability assay, > 99% inactivated after 10 days at 37 degrees C, 4 days at 47 degrees C, and 2 days at 55 degrees C. Eggs were more rapidly inactivated in anaerobic digesters than in aerobic digesters. At 55 degrees C, eggs in both anaerobic and aerobic digesters were > 99% inactivated within 1 h. At 47 degrees C, anaerobic digestion inactivated around 95% eggs in 2 days, but around 25% of the eggs were still viable after 10 days in aerobic digesters. At 37 degrees C, anaerobic digestion inactivated more than 75% of the eggs after 10 days, but in the aerobic digester at 37 degrees C, 10 days of treatment had no effect on viability. The oocysts and eggs added in chambers appeared to behave similarly to these pathogens added directly to the biosolids within the digesters.
对接种到好氧和厌氧消化器中的微小隐孢子虫卵囊和猪蛔虫卵的生存能力进行了测定。消化器维持在37℃、47℃和55℃，停留时间为10天。虫卵和卵囊以单次加样的方式添加到每个消化器中，或以放置在消化器中的隔室形式添加不同时间段。通过染料渗透性试验确定，在所有系统中卵囊均迅速失活，在37℃下10天后、47℃下4天后和55℃下2天后，失活率均>99%。虫卵在厌氧消化器中的失活速度比在好氧消化器中更快。在55℃下，厌氧和好氧消化器中的虫卵在1小时内失活率均>99%。在47℃下，厌氧消化在2天内使约95%的虫卵失活，但在好氧消化器中10天后仍有约25%的虫卵存活。在37℃下，厌氧消化10天后使超过75%的虫卵失活，但在37℃的好氧消化器中，10天的处理对生存能力没有影响。添加在隔室中的卵囊和虫卵的行为似乎与直接添加到消化器内生物固体中的这些病原体相似。
