Qiao Chuan-Ling, Yu Kang-Zhen, Jiang Yong-Ping, Jia Yong-Qing, Tian Guo-Bin, Liu Ming, Deng Guo-Hua, Wang Xiu-Rong, Meng Qing-Wen, Tang Xiu-Ying
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, P.R. China.
Avian Pathol. 2003 Feb;32(1):25-32. doi: 10.1080/0307945021000070688.
Inactivated whole avian influenza virus (AIV) vaccine provides protection against homologous haemagglutinin (HA) subtype virus, but poor protection against a heterologous HA virus. Moreover, it induces chickens to produce antibodies to cross-reactive antigens, especially nucleoprotein, which is limits AIV serological surveillance. In this study, a recombinant fowlpox virus co-expressing HA (H5 subtype) and NA (NI subtype)genes of AIV was evaluated for its ability to protect chickens against intramuscular challenge with a lethal dose of highly pathogenic (HP) AIV. Susceptible chickens were also vaccinated by wing-web puncture with the parent fowlpox vaccine virus. Following challenge 4 weeks later with HPAIV, all chickens vaccinated with recombinant virus were protected, while the chickens vaccinated with either the unaltered parent fowlpox vaccine virus or unvaccinated controls experienced 100% mortality following challenge. This protection was accompanied by the high levels of specific antibody to the respective components of the recombinant vaccine. The above results showed that rFPV-HA-NA could be a potential vaccine to replace current inactivated vaccines for preventing AI.
灭活全禽流感病毒（AIV）疫苗可提供针对同源血凝素（HA）亚型病毒的保护，但对异源HA病毒的保护作用较差。此外，它会诱导鸡产生针对交叉反应抗原的抗体，尤其是核蛋白，这限制了AIV的血清学监测。在本研究中，评估了一种共表达AIV的HA（H5亚型）和NA（N1亚型）基因的重组禽痘病毒保护鸡免受致死剂量高致病性（HP）AIV肌肉注射攻击的能力。易感鸡也通过翼膜穿刺接种亲本禽痘疫苗病毒。4周后用HPAIV攻击，所有接种重组病毒的鸡都受到保护，而接种未改变的亲本禽痘疫苗病毒或未接种的对照鸡在攻击后死亡率为100%。这种保护伴随着针对重组疫苗各组分的高水平特异性抗体。上述结果表明，rFPV-HA-NA可能是一种潜在的疫苗，可替代目前的灭活疫苗来预防禽流感。
