Morton C Oliver, Mauchline Tim H, Kerry Brian R, Hirsch Penny R
Nematode Interactions Unit, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK.
Mycol Res. 2003 Feb;107(Pt 2):198-205. doi: 10.1017/s0953756203007251.
The mitosporic fungus Pochonia chlamydosporia is a potential biocontrol agent for cyst (Heterodera spp. and Globodera spp.) and root knot (Meloidogyne spp.) nematodes, which are important agricultural plant pests. 54 isolates from diverse geographical regions and several nematode hosts were used in this study. Genetic variation was examined using enterobacterial repetitive intergenic consensus (ERIC) primed PCR and sequences from the internal transcribed spacer (ITS) rRNA region. ERIC PCR yielded 35 scorable binary characters from all the fungi tested and cluster analysis of the data showed that isolates from cyst nematodes were more genetically variable than those from root knot nematodes. The ITS regions were highly conserved, the only significant difference being an extra thymidine in isolates from Meloidogyne spp. Assays with nematode eggs indicated that isolates differ in their ability to infect different nematode genera. The results indicate host related variation in P. chlamydosporia. This finding has significant implications for the application of P. chlamydosporia as a biocontrol agent.
丝孢菌厚垣孢普可尼亚菌是一种潜在的生物防治剂，可用于防治孢囊线虫（异皮线虫属和球孢囊线虫属）和根结线虫（根结线虫属），这些都是重要的农业植物害虫。本研究使用了来自不同地理区域和几种线虫寄主的54个分离株。利用肠杆菌重复基因间共有序列（ERIC）引发的PCR和内部转录间隔区（ITS）rRNA区域的序列检测遗传变异。ERIC-PCR从所有测试真菌中产生了35个可评分的二元特征，数据聚类分析表明，来自孢囊线虫的分离株比来自根结线虫的分离株具有更高的遗传变异性。ITS区域高度保守，唯一显著的差异是根结线虫属分离株中有一个额外的胸腺嘧啶。对线虫卵的检测表明，不同分离株感染不同线虫属的能力存在差异。结果表明厚垣孢普可尼亚菌存在寄主相关变异。这一发现对厚垣孢普可尼亚菌作为生物防治剂的应用具有重要意义。
