Gu Min, Bird Damian
Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering, Swinburne University of Technology, P.O. Box 218, Hawthorn 3122, Victoria, Australia.
J Opt Soc Am A Opt Image Sci Vis. 2003 May;20(5):941-7. doi: 10.1364/josaa.20.000941.
The three-dimensional optical transfer function is derived for analyzing the imaging performance in fiber-optical two-photon fluorescence microscopy. Two types of fiber-optical geometry are considered: The first involves a single-mode fiber for delivering a laser beam for illumination, and the second is based on the use of a single-mode fiber coupler for both illumination delivery and signal collection. It is found that in the former case the transverse and axial cutoff spatial frequencies of the three-dimensional optical transfer function are the same as those in conventional two-photon fluorescence microscopy without the use of a pinhole.However, the transverse and axial cutoff spatial frequencies in the latter case are 1.7 times as large as those in the former case. Accordingly, this feature leads to an enhanced optical sectioning effect when a fiber coupler is used, which is consistent with our recent experimental observation.
推导了三维光学传递函数，用于分析光纤双光子荧光显微镜的成像性能。考虑了两种光纤几何结构：第一种涉及用于传输激光束进行照明的单模光纤，第二种基于使用单模光纤耦合器进行照明传输和信号采集。发现在前一种情况下，三维光学传递函数的横向和轴向截止空间频率与不使用针孔的传统双光子荧光显微镜中的相同。然而，后一种情况下的横向和轴向截止空间频率是前一种情况下的1.7倍。因此，当使用光纤耦合器时，这一特性导致增强的光学切片效果，这与我们最近的实验观察结果一致。
