Bird Damian, Gu Min
Opt Lett. 2002 Jun 15;27(12):1031-3. doi: 10.1364/ol.27.001031.
We present a two-photon fluorescence microscope based on a three-port single-mode optical fiber coupler. It is found that the coupler behaves as a low-pass filter that can deliver an ultrashort-pulsed laser beam of as much as 150 mW of power in the wavelength range from 770 to 870 nm as well as collect a two-photon fluorescence signal in the visible range. As a result of using the fiber coupler, the new two-photon imaging system exhibts a number of advantages, including a compact arrangement, freedom from vibration from lasers and electronic devices, self-alignment, reduction of multiple scattering, and an enhanced optical sectioning effect. The effectiveness of the new instrument is demonstrated with a set of three-dimensional images of biological samples. This instrument may make two-photon fluorescence endoscopy possible for in vivo medical applications.
我们展示了一种基于三端口单模光纤耦合器的双光子荧光显微镜。研究发现，该耦合器表现为一个低通滤波器，能够在770至870纳米波长范围内传输功率高达150毫瓦的超短脉冲激光束，同时收集可见范围内的双光子荧光信号。由于使用了光纤耦合器，新型双光子成像系统具有诸多优点，包括结构紧凑、不受激光和电子设备振动影响、自对准、减少多重散射以及增强光学切片效果。通过一组生物样品的三维图像证明了该新型仪器的有效性。该仪器可能使双光子荧光内窥镜用于体内医学应用成为可能。
