Kropp J, Weicker H
Z Immunitatsforsch Exp Klin Immunol. 1975 Sep;150(3):267-76.
Incubation of Rh positive ghosts with phospholipase A2 and C abolished the adsorption of Rh antibodies on the ghosts; incubation with phospholipase D, however, did not affect their adsorption and none of these phospholipases affected the adsorption of antibodies of the ABO system. The impairment of antigen-antibody-reaction in Rh positive ghosts treated with phospholipase corresponds to the absence of the antigen-antibody reaction with the membrane protein associated with Rh characteristics in the Schultz-Dale-Test. The chromatogram of the phospholipids extracted from those stromata treated with various phospholipases and those not treated showed different patterns. After incubation with phospholipase-A2 the lecithin and cephalin streaks were reduced and in addition lysophosphatide and fatty acid streaks were detected. In the case of phospholipase C the lecithin and cephalin streaks were further reduced while diglyceride streaks made their appearance. The phospholipid extracts from those stromata treated with phospholipase D and those not treated were identical. Phospholipase C reduced the values of lipid phosphorus more than did phospholipase A2, while phospholipase D did not reduce them at all. This study supports the results of other investigators who have postulated that the Rh antigens are located in a lipoprotein on the membrane of the human erythrocyte. The antigen-antibody-reaction seems to require a precise protein-phospholipid interaction.
用磷脂酶A2和C孵育Rh阳性血影可消除Rh抗体在血影上的吸附；然而，用磷脂酶D孵育并不影响其吸附，且这些磷脂酶均不影响ABO系统抗体的吸附。用磷脂酶处理的Rh阳性血影中抗原 - 抗体反应的受损与舒尔茨 - 戴尔试验中与Rh特性相关的膜蛋白的抗原 - 抗体反应缺失相对应。从用各种磷脂酶处理和未处理的那些基质中提取的磷脂色谱图显示出不同的模式。用磷脂酶 - A2孵育后，卵磷脂和脑磷脂条带减少，此外还检测到溶血磷脂和脂肪酸条带。在磷脂酶C的情况下，卵磷脂和脑磷脂条带进一步减少，同时甘油二酯条带出现。用磷脂酶D处理和未处理的那些基质的磷脂提取物是相同的。磷脂酶C比磷脂酶A2更能降低脂质磷的值，而磷脂酶D根本不会降低它们。这项研究支持了其他研究者的结果，他们推测Rh抗原位于人红细胞膜上的一种脂蛋白中。抗原 - 抗体反应似乎需要精确的蛋白质 - 磷脂相互作用。
