Barnes R M, Duguid J K, Roberts F M, Risk J M, Johnson P M, Finn R, Hardy J, Napier J A, Clarke C A
Clin Exp Immunol. 1987 Jan;67(1):220-6.
The effects of prior oral administration of erythrocyte membrane preparations (Oral Rh antigen) on the serum anti-Rh(D) antibody response has been evaluated in non-sensitized Rh(D)-negative male volunteers, and in female volunteers sensitized previously by Rh(D)-positive fetal blood during pregnancy. Sixty-one percent (11/18) of males who received oral Rh antigen (either D-positive or D-negative) before intravenous challenge with Rh(D)-positive cells produced detectable antibodies; of these 11, six received oral Rh(D)-negative antigen and five received oral Rh(D)-positive antigen. Seventy-two percent (13/18) of control males, who had received no prior oral Rh antigen, produced antibodies following challenge with Rh(D)-positive cells. Three out of six pre-sensitized females who received oral D-positive or D-negative Rh antigen for 4 weeks, but without intravenous challenge, increased their anti-Rh(D) antibody levels which peaked after 11-18 weeks: two had received Rh(D)-positive antigen, and one Rh(D)-negative antigen. These data indicate that administration of oral Rh antigen before parenteral immunization does not significantly suppress the anti-Rh(D) antibody response. Indeed, oral administration of either Rh(D)-positive or Rh(D)-negative antigen can boost systemic antibody in pre-sensitized females. These results do not support the rationale of treating Rh-sensitized pregnant women with oral Rh antigen.
在未致敏的Rh（D）阴性男性志愿者以及孕期曾因Rh（D）阳性胎儿血液致敏的女性志愿者中，评估了预先口服红细胞膜制剂（口服Rh抗原）对血清抗Rh（D）抗体反应的影响。在静脉注射Rh（D）阳性细胞之前接受口服Rh抗原（D阳性或D阴性）的男性中，61%（11/18）产生了可检测到的抗体；在这11人中，6人接受了口服Rh（D）阴性抗原，5人接受了口服Rh（D）阳性抗原。未预先口服Rh抗原的对照男性中，72%（13/18）在接受Rh（D）阳性细胞攻击后产生了抗体。6名预先致敏的女性中有3人接受了4周的口服D阳性或D阴性Rh抗原，但未进行静脉注射攻击，她们的抗Rh（D）抗体水平有所升高，在11 - 18周后达到峰值：2人接受了Rh（D）阳性抗原，1人接受了Rh（D）阴性抗原。这些数据表明，在胃肠外免疫之前给予口服Rh抗原不会显著抑制抗Rh（D）抗体反应。实际上，口服Rh（D）阳性或Rh（D）阴性抗原均可增强预先致敏女性的全身抗体。这些结果不支持用口服Rh抗原治疗Rh致敏孕妇的理论依据。
