用于从人类粪便中分离阿尔堡短螺旋体的选择性培养基的评估。
Evaluation of selective media for the isolation of Brachyspira aalborgi from human faeces.
作者信息
Brooke C Josephine, Riley Thomas V, Hampson David J
机构信息
Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia 2,3Division of Microbiology and Infectious Diseases, The Western Australian Centre of Pathology and Medical Research2 and Department of Microbiology, The University of Western Australia3, Nedlands, Western Australia 6009, Australia#dReceived 5 November 2002 Accepted 2 March 2003.
出版信息
J Med Microbiol. 2003 Jun;52(Pt 6):509-513. doi: 10.1099/jmm.0.05105-0.
The purposes of this study were to identify a solid medium that supports improved growth of the anaerobic intestinal spirochaete Brachyspira aalborgi, to modify this for use as a selective isolation medium and then to test the medium for its effectiveness in isolating B. aalborgi from patients' faeces. Of the media evaluated, brain heart infusion agar (BHIA) with 10 % bovine blood (BB) was the most effective base-supplement combination for growth, with colonies attaining 1.2 mm in diameter by 21 days. Incubation in an anaerobic jar (94 % H(2), 6 % CO(2)) permitted growth of larger colonies than incubation in an anaerobic chamber (80 % N(2), 10 % H(2), 10 % CO(2)). Growth was improved only slightly at 38.5 degrees C compared with 37 degrees C. Selection of B. aalborgi from artificially seeded faeces was achieved equally well on eight different solid media containing spectinomycin (400 micro g ml(-1)) alone or in combinations with polymyxin B (5 micro g ml(-1)), colistin (25 micro g ml(-1)) and rifampicin (12.5 micro g ml(-1)). By using BHIA 10 % BB with spectinomycin plus polymyxin B, B. aalborgi was isolated from one of five human faecal samples that were positive for B. aalborgi by PCR amplification. This is the first report of the isolation of B. aalborgi from human faeces.
本研究的目的是确定一种能促进厌氧肠道螺旋体阿氏短螺旋体生长的固体培养基，对其进行改良以用作选择性分离培养基，然后测试该培养基从患者粪便中分离阿氏短螺旋体的有效性。在所评估的培养基中，含10%牛血（BB）的脑心浸液琼脂（BHIA）是最有利于生长的基础培养基 - 添加物组合，21天时菌落直径可达1.2毫米。在厌氧罐（94% H₂，6% CO₂）中培养比在厌氧箱（80% N₂，10% H₂，10% CO₂）中培养能形成更大的菌落。与37℃相比，在38.5℃下生长仅略有改善。在单独含有壮观霉素（400 μg ml⁻¹）或与多粘菌素B（5 μg ml⁻¹）、黏菌素（25 μg ml⁻¹）和利福平（12.5 μg ml⁻¹）组合的八种不同固体培养基上，从人工接种粪便中筛选阿氏短螺旋体的效果相同。通过使用含壮观霉素加多粘菌素B的10% BB的BHIA，从通过PCR扩增检测出阿氏短螺旋体呈阳性的五份人类粪便样本中的一份中分离出了阿氏短螺旋体。这是首次从人类粪便中分离出阿氏短螺旋体的报告。
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