Cho Sae-Youll, Payne Gregory T, Brown Thomas M
Genesis. 2003 May;36(1):34-9. doi: 10.1002/gene.10191.
Black eyes of the moth of Heliothis virescens were controlled by a single, autosomal recessive gene, b. Black-eyed moths were discovered among progeny in an outcross made to test for allelism of two known genes ye, conferring yellow eyes, and yes, conferring yellow eyes and scales. Complementation to the wildtype gray eye color was observed in 686 (99.1%) of the progeny; however, six progeny of one mating exhibited the new phenotype, black eyes. Two black-eyed females mated to a wildtype sibling produced descendents displaying golden eyes, striped eye, purple eyes, white eyes, and "cat's" eyes. No black-eyed progeny were observed in the F2 generation of lines segregating for y, ye, and yes, confirming that black eye was not a combination of those other genes. These newly discovered genes could be useful in basic studies of developmental genetics or in applied transgenesis.
烟芽夜蛾的黑眼由一个常染色体隐性基因b控制。在一次用于测试两个已知基因（ye，导致黄眼；yes，导致黄眼和鳞片）等位性的远交后代中发现了黑眼蛾。在686只（99.1%）后代中观察到了与野生型灰眼颜色的互补；然而，一次交配的6只后代表现出了新的表型——黑眼。两只黑眼雌蛾与一只野生型同胞交配产生的后代表现出金眼、条纹眼、紫眼、白眼和“猫眼”。在分离y、ye和yes的品系的F2代中未观察到黑眼后代，这证实黑眼不是那些其他基因的组合。这些新发现的基因可能在发育遗传学的基础研究或应用转基因研究中有用。
