Huang Ray-Ling, Huang Yu-Ling, Ou Jun-Chih, Chen Chien-Chih, Hsu Feng-Lin, Chang Chungming
National Research Institute of Chinese Medicine, No. 155-1, Sec. 2, Li Nung St., Peitou, Taipei, Taiwan, Republic of China.
Phytother Res. 2003 May;17(5):449-53. doi: 10.1002/ptr.1167.
Using an HBV-producing cell line and inhibition of the expression of the HBsAg and HBeAg as antiviral indicators, a study was conducted on 25 compounds isolated from four Phyllanthus (Euphorbiaceae) plants, including P. amarus Schum. & Thonn., P. multi florus Willd., P. tenellus Roxb. and P. virgatus Forst. f. It was found that niranthin (1), nirtetralin (3), hinokinin (5) and geraniin (13) at the non-cytotoxic concentration of 50 micro m, suppressed effectively both HBsAg and HBeAg expression, with the highest inhibition at 74.3%, 45.3%; 69.6%, 33.9%; 68.1%, 52.3%; 32.1%, 46.6%, respectively. Of these, niranthin (1) showed the best anti-HBsAg activity, while the most potent anti-HBeAg activity was observed with hinokinin (5).
以一种产生乙肝病毒的细胞系以及抑制乙肝表面抗原（HBsAg）和乙肝e抗原（HBeAg）的表达作为抗病毒指标，对从四种叶下珠属（大戟科）植物中分离得到的25种化合物进行了研究，这四种植物包括苦味叶下珠、多花叶下珠、纤细叶下珠和线叶叶下珠。研究发现，在50微摩尔的非细胞毒性浓度下，尼润素（1）、尼润特林（3）、扁柏双黄酮（5）和老鹳草素（13）能有效抑制HBsAg和HBeAg的表达，抑制率最高分别为74.3%、45.3%；69.6%、33.9%；68.1%、52.3%；32.1%、46.6%。其中，尼润素（1）表现出最佳的抗HBsAg活性，而扁柏双黄酮（5）的抗HBeAg活性最强。
