Sandgren Katarina, Lin Zhong, Fex Svenningsen Asa, Ekblad Eva
Department of Physiological Sciences, Neuroendocrine Cell Biology, Lund University, Lund, Sweden.
J Neurosci Res. 2003 Jun 1;72(5):595-602. doi: 10.1002/jnr.10612.
Several motility disorders originate in the enteric nervous system (ENS). Our knowledge of factors governing survival of the ENS is poor. Changes in the expression of vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) in enteric neurons occur after neuronal injury and in intestinal adaptation. The aim of this study was to evaluate whether VIP and nitric oxide (NO) influence survival of cultured, dissociated myenteric neurons. Neuronal survival was evaluated after 0, 4, and 8 days in culture. Influence of VIP and NO on neuronal survival was examined after culturing in the presence of VIP, NO donor, VIP antiserum, or NOS inhibitor. A marked loss of neurons was noted during culturing. VIP and NO significantly promoted neuronal survival. Corroborating this was the finding of an enhanced neuronal cell loss when cultures were grown in the presence of VIP antiserum or NOS inhibitor.
几种运动障碍起源于肠神经系统（ENS）。我们对支配ENS存活的因素了解甚少。在神经元损伤后以及肠道适应过程中，肠神经元中血管活性肠肽（VIP）和一氧化氮合酶（NOS）的表达会发生变化。本研究的目的是评估VIP和一氧化氮（NO）是否影响培养的、解离的肌间神经元的存活。在培养0、4和8天后评估神经元的存活情况。在存在VIP、NO供体、VIP抗血清或NOS抑制剂的情况下培养后，检测VIP和NO对神经元存活的影响。在培养过程中发现神经元明显减少。VIP和NO显著促进了神经元的存活。当在VIP抗血清或NOS抑制剂存在的情况下培养时，神经元细胞损失增加，这一发现证实了上述结果。
