金属离子对黄曲霉生长、基因表达及黄曲霉毒素合成的增强作用：逆转录聚合酶链反应表征
Metal ion enhancement of fungal growth, gene expression and aflatoxin synthesis in Aspergillus flavus: RT-PCR characterization.
作者信息
Cuero R, Ouellet T, Yu J, Mogongwa N
机构信息
Prairie View A & M University, CARC, PO BOX 685, Prairie View, TX 77446, USA.
出版信息
J Appl Microbiol. 2003;94(6):953-61. doi: 10.1046/j.1365-2672.2003.01870.x.
AIMS
To determine the effect of mineral ions (e.g., Zn2+, Cu2+, and Fe2+) on the enhancement of fungal growth, total RNA, aflatoxin pathway gene expression, and production of aflatoxin and its precursor O-methylsterigmatocystin (OMST).
METHODS AND RESULTS
The influence of the metal ions, as a single or mixed treatments, was observed in submerged cultures of toxigenic Aspergillus flavus through changes in the fungal RNA or aflatoxin pathway gene (omtA) by reverse transcription-polymerase chain reaction (RT-PCR) levels, and also in fungal dry-weight accumulation, aflatoxin and OMST production.
CONCLUSIONS
The ion treatments induced changes of fungal total RNA, mRNA levels, associate fungal growth, biosynthesis of aflatoxin and OMST, and enhanced expression of RT-PCR.
SIGNIFICANCE AND IMPACT OF STUDY
Demonstrates at the cellular and molecular level, the significant effects of metal ions on both fungal growth and production of aflatoxin.
目的
确定矿物质离子（如锌离子、铜离子和亚铁离子）对促进真菌生长、总RNA、黄曲霉毒素途径基因表达以及黄曲霉毒素及其前体O-甲基柄曲霉素（OMST）产生的影响。
方法与结果
通过逆转录聚合酶链反应（RT-PCR）水平观察真菌RNA或黄曲霉毒素途径基因（omtA）的变化，以及在产毒黄曲霉的深层培养中，以单一或混合处理方式观察金属离子的影响，同时观察真菌干重积累、黄曲霉毒素和OMST的产生情况。
结论
离子处理诱导了真菌总RNA、mRNA水平的变化，影响了真菌生长、黄曲霉毒素和OMST的生物合成，并增强了RT-PCR的表达。
研究的意义与影响
在细胞和分子水平上证明了金属离子对真菌生长和黄曲霉毒素产生的显著影响。
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