Shaukat S S, Siddiqui I A
Soil Biology and Ecology Laboratory, Department of Botany, University of Karachi, Karachi, Pakistan.
Lett Appl Microbiol. 2003;36(6):392-8. doi: 10.1046/j.1472-765x.2003.01325.x.
To determine the influence of various trace minerals and carbon source on the biocontrol performance of Pseudomonas aeruginosa strain IE-6S+ and P. fluorescens strain CHA0 against Macrophomina phaseolina.
In dual culture plate assay, P. aeruginosa IE-6S+ and P. fluorescens CHA0 inhibited radial growth of M. phaseolina producing zones of inhibition. Czapek's dox agar medium amended with both zinc and glucose remarkably improved antifungal activities of the bacterial inoculants. Under glasshouse conditions, soil amendment with zinc and/or glucose alone did not reduce M. phaseolina infection in tomato roots but did reduce significantly when used in combination with IE-6S+ or CHA0. Soil amendments with zinc and/or glucose increased fresh shoot weights but zinc amendment greatly reduced bacterial populations in the rhizosphere.
Mineral and carbon amendments enhance the biocontrol performance of fluorescent pseudomonads against M. phaseolina.
Identification of mineral and carbon amendments that favour biocontrol of certain bacterial strains may provide clues to soil factors or components of nutrient solutions in hydroponic culture that will improve the level and reliability of control.
确定各种微量矿物质和碳源对铜绿假单胞菌菌株IE-6S+和荧光假单胞菌菌株CHA0防治菜豆壳球孢菌性能的影响。
在双培养平板试验中，铜绿假单胞菌IE-6S+和荧光假单胞菌CHA0抑制了菜豆壳球孢菌的径向生长，产生了抑菌圈。添加锌和葡萄糖的查氏琼脂培养基显著提高了接种细菌的抗真菌活性。在温室条件下，单独用锌和/或葡萄糖改良土壤并不能减少番茄根中菜豆壳球孢菌的感染，但与IE-6S+或CHA0联合使用时则能显著减少。用锌和/或葡萄糖改良土壤增加了地上部鲜重，但锌改良显著减少了根际细菌数量。
矿物质和碳源改良增强了荧光假单胞菌对菜豆壳球孢菌的生防性能。
确定有利于某些细菌菌株生防的矿物质和碳源改良剂，可能为土壤因素或水培营养液成分提供线索，从而提高防治水平和可靠性。
