Mackay Duncan J D, Moyer Kurtis E, Saggers Gregory C, Myers Roland L, Mackay Donald R, Ehrlich H Paul
I. S. Zubar Wound Healing Laboratory, Division of Plastic Surgery and Department of Neurosciences and Anatomy, Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
Wound Repair Regen. 2003 May-Jun;11(3):204-12. doi: 10.1046/j.1524-475x.2003.11309.x.
Systemic ingestion of vanadate, a nonspecific inhibitor of tyrosine phosphatases, doubles wound breaking strength, enhances the packing of collagen fibers, and prevents the appearance of myofibroblasts in granulation tissue. Will the local application of vanadate mimic the systemic effects? Pairs of polyvinyl alcohol sponges, each with a central reservoir and attached injection port, were subcutaneously implanted in rats. Daily, one implant received 0.2 ml of saline and the other received 0.2 ml of 0.03 mM vanadate in saline. On day 7, harvested sponges had equivalent wet weights. The vanadate-treated sponges had fibroblasts separated by connective tissue, with a more intense birefringence of the collagen fibers. Transmission electron microscopy showed collagen more uniformly packed in the vanadate treated sponges where collagen fibers were equally spaced and had equal diameters. By immunohistology, myofibroblasts, defined by the expression of alpha-smooth muscle actin within stress fibers, were absent in vanadate-treated granulation tissue. The expression of alpha-smooth muscle actin was restricted to smooth muscle cells of blood vessels. Controls had densely packed alpha-smooth muscle actin staining myofibroblasts, weak birefringence, and randomly spaced collagen fibers with irregular diameters. We conclude that the local application of vanadate prevents the appearance of myofibroblasts and optimizes the organization of collagen fibers in developing granulation tissue.
酪氨酸磷酸酶的非特异性抑制剂钒酸盐经全身摄入后，可使伤口断裂强度加倍，增强胶原纤维的排列，并防止肉芽组织中出现肌成纤维细胞。局部应用钒酸盐会产生与全身应用类似的效果吗？将成对的聚乙烯醇海绵（每个海绵都有一个中央储液器和一个连接的注射端口）皮下植入大鼠体内。每天，一个植入物接受0.2 ml生理盐水，另一个接受0.2 ml含0.03 mM钒酸盐的生理盐水。在第7天，收获的海绵湿重相当。用钒酸盐处理过的海绵中的成纤维细胞被结缔组织分隔开，胶原纤维的双折射更强。透射电子显微镜显示，在经钒酸盐处理的海绵中，胶原排列更均匀，胶原纤维间距相等且直径相同。通过免疫组织学方法发现，在经钒酸盐处理的肉芽组织中，由应力纤维内α平滑肌肌动蛋白表达所定义的肌成纤维细胞不存在。α平滑肌肌动蛋白的表达仅限于血管平滑肌细胞。对照组的肌成纤维细胞α平滑肌肌动蛋白染色密集，双折射弱，胶原纤维间距随机且直径不规则。我们得出结论，局部应用钒酸盐可防止肌成纤维细胞出现，并优化正在形成的肉芽组织中胶原纤维的组织排列。
