Division of Plastic Surgery, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.
Ann N Y Acad Sci. 2010 Apr;1194:118-24. doi: 10.1111/j.1749-6632.2010.05483.x.
Incisional wounds in rats treated locally with thymosin beta4 (Tbeta4) healed with minimal scaring and without loss in wound breaking strength. Treated wounds were significantly narrower in width. Polarized light microscopy treated wounds had superior organized collagen fibers, displaying a red birefringence, which is consistent with mature connective tissue. Control incisions had randomly organized collagen fibers, displaying green birefringence that is consistent with immature connective tissue. Immunohistology treated wounds had few myofibroblasts and fibroblasts with alpha smooth muscle actin (SMA) stained stress fibers. Polyvinyl alcohol sponge implants placed in subcutaneous pockets received either carrier or 100 microg of Tbeta4 on days 2, 3, and 4. On day 14, treated implants revealed longer, thicker collagen fiber bundles with intense yellow-red birefringence by polarized light microscopy. In controls, fine, thin collagen fiber bundles were arranged in random arrays with predominantly green birefringence. Controls contained mostly myofibroblasts, while few myofibroblasts appeared in Tbeta4 treated implants. Electron microscopy confirmed both cell types and the degree of collagen fiber bundle organization. Our results demonstrate that Tbeta4 treated wounds appear to mature earlier and heal with minimal scaring.
经局部给予胸腺肽β4（Tβ4）治疗的大鼠切口愈合后，瘢痕最小，且伤口断裂强度无损失。治疗后的伤口明显变窄。偏光显微镜下，治疗后的伤口胶原纤维排列更有序，呈现红色双折射，与成熟的结缔组织一致。对照切口的胶原纤维排列杂乱，呈现绿色双折射，与不成熟的结缔组织一致。免疫组织化学染色显示，治疗后的伤口肌成纤维细胞和纤维母细胞较少，α平滑肌肌动蛋白（SMA）染色的应激纤维。聚乙烯醇海绵植入物放置在皮下袋中，第 2、3 和 4 天分别给予载体或 100μg Tβ4。第 14 天，经治疗的植入物通过偏光显微镜显示出更长、更厚的胶原纤维束，具有强烈的黄-红色双折射。在对照组中，细而薄的胶原纤维束呈随机排列，主要呈绿色双折射。对照组主要含有肌成纤维细胞，而 Tβ4 治疗的植入物中肌成纤维细胞较少。电子显微镜证实了这两种细胞类型和胶原纤维束的组织程度。我们的结果表明，Tβ4 治疗的伤口似乎更早成熟，瘢痕最小。
