Cagli S, Oktar N, Dalbasti T, Erensoy S, Ozdamar N, Göksel S, Sayiner A, Bilgiç A
Department of Neurosurgery, Ege University School of Medicine, Izmir, Turkey.
J Neurol Neurosurg Psychiatry. 2003 Jun;74(6):756-9. doi: 10.1136/jnnp.74.6.756.
Chlamydia pneumoniae (C pneumoniae) is a common cause of a usually mild, community acquired pneumonia. This organism, however, can spread from the respiratory tract into other parts of the body and has been detected in up to 70% of atheromatous lesions in blood vessels. Although the exact mechanism of the C Pneumoniae contribution to the pathogenesis of atherosclerosis remains unknown, prophylactic antibiotic trials are planned for people at high risk for coronary disease.
In this study the authors aimed to investigate C pneumoniae DNA content in the cerebral aneurysmal sac tissue with the aid of polymerase chain reaction (PCR) method. C pneumoniae DNA was searched in 15 surgically clipped and removed aneurysmal sac tissue and in two tumour (an ependymoma of the fourth ventricle and a craniofaringoma) samples by touchdown enzyme time release PCR (TETR PCR) targeting 16S rRNA gene and by nested PCR targeting ompA gene.
Both PCR methods were sensitive to detect in C pneumoniae 4x10(-2) genomes. C pneumoniae DNA was not detected in any of the 17 sample tissues of these patients.
The contribution of C pneumoniae in the development of intracranial aneurysms cannot be excluded despite the results of this study. Further studies on the possible role of C pneumoniae or any other micro-organisms in the pathogenesis of aneurysms should be performed.
肺炎衣原体（C肺炎衣原体）是通常为轻症的社区获得性肺炎的常见病因。然而，这种病原体可从呼吸道扩散至身体其他部位，并且在高达70%的血管粥样硬化病变中被检测到。尽管C肺炎衣原体在动脉粥样硬化发病机制中的具体作用机制尚不清楚，但已计划针对冠心病高危人群开展预防性抗生素试验。
在本研究中，作者旨在借助聚合酶链反应（PCR）方法研究脑动脉瘤瘤囊组织中的C肺炎衣原体DNA含量。通过针对16S rRNA基因的降落酶定时释放PCR（TETR PCR）和针对ompA基因的巢式PCR，在15个手术夹闭并切除的动脉瘤瘤囊组织以及两个肿瘤（第四脑室室管膜瘤和颅咽管瘤）样本中搜索C肺炎衣原体DNA。
两种PCR方法对检测4×10⁻²个基因组的C肺炎衣原体均敏感。在这些患者的17个样本组织中均未检测到C肺炎衣原体DNA。
尽管本研究有此结果，但不能排除C肺炎衣原体在颅内动脉瘤发生过程中的作用。应进一步研究C肺炎衣原体或任何其他微生物在动脉瘤发病机制中的可能作用。
