Taylor R G, Fowler W M
Arch Phys Med Rehabil. 1976 May;57(5):223-8.
Simultaneous study of the function of fast and slow twitch skeletal muscles in the same in vitro preparation has obvious advantages. Equipment, method and technique are described for study of the soleus (slow) and extensor digitorum longus (fast) muscles from the mouse, and the problems involved in preparing and standardizing these studies are discussed. For nearly maximum function to be obtained from both muscles, careful attention must be given to the stimulus parameters used. Three separate studies were necessary to determine the appropriate stimulus duration, frequency and rest interval between the stimulus trains. The results indicate that for study of tetanic tension and rate of tension development of these muscles from the C57 NAB strain of mice at 20C the most satisfactory stimulus parameters are : duration 2 msec, frequency 100 pulses per second and duration of the stimulus train 700 msec. Rest interval between stimulus trains was two minutes as problems developed with rest intervals less than two minutes or greater than four minutes.
在同一体外制备物中同时研究快肌和慢肌的功能具有明显优势。本文描述了用于研究小鼠比目鱼肌（慢肌）和趾长伸肌（快肌）的设备、方法和技术，并讨论了进行这些研究时在制备和标准化过程中涉及的问题。为了使两块肌肉都能获得近乎最大功能，必须仔细关注所使用的刺激参数。需要进行三项独立研究来确定合适的刺激持续时间、频率以及刺激串之间的休息间隔。结果表明，对于在20℃下研究C57 NAB品系小鼠的这些肌肉的强直张力和张力发展速率而言，最令人满意的刺激参数是：持续时间2毫秒，频率每秒100个脉冲，刺激串持续时间700毫秒。当休息间隔小于两分钟或大于四分钟时会出现问题，因此刺激串之间的休息间隔为两分钟。
