Yang Jian, Zhao Jingbo, Zeng Yanjun, Vinter-Jensen Lars, Gregersen Hans
Beijing Polytechnic University, Beijing, China.
Dig Dis Sci. 2003 Mar;48(3):442-8. doi: 10.1023/a:1022516111538.
Systemic treatment with epidermal growth factor (EGF) induce growth of the large intestine. The aim of this study was to investigate the morphological properties early in the course of EGF-induced large intestinal growth. The effects of systemic EGF treatment on the morphological properties at the zero-stress state along the large intestine were investigated. EGF-treated rats and control rats were allocated into group with EGF treatment for 2, 4, 7, and 14 days (N = 6 for each EGF treatment group except N = 4 for the 14-day group). The controls had saline injected (N = 3 for each group). The excised large intestine was subdivided into four segments: the ascending colon, transverse colon, descending colon, and rectum. The length and weight of each segment was measured. The zero-stress state was obtained by cutting rings of the large intestine radially, and the opening angle was measued on video images. The thickness and cross-sectional area of the mucosa and muscle layers, and the inner and outer circumference were measured. The total colon length did not increase, whereas the weight of the large intestine, muscosal thickness and mass, and inner and outer circumference increased significantly (P < 0.05). The increase was most prominent in the proximal colon after 7 days of EGF treatment. Later no further morphological changes were observed, except for a decrease in mucosal thickness in most segments and in mucosal cross-sectional area in the descending colon. In the controls and during the first week of EGF treatment, the opening angle was approximately 100 degrees. After 14 days the opening angle increased significantly in the ascending and transverse colon to approximately 172 and 135 degrees (P < 0.05). In conclusion, this study demonstrates that systemic EGF treatment caused remodeling of the morphology of the zero-stress state in the large intestine in a time-dependent manner. The growth was most pronounced in the ascending and transverse colon and involved mainly the mucosal layer.
用表皮生长因子（EGF）进行全身治疗可诱导大肠生长。本研究的目的是调查EGF诱导大肠生长过程早期的形态学特性。研究了全身EGF治疗对大肠零应力状态下形态学特性的影响。将接受EGF治疗的大鼠和对照大鼠分为接受EGF治疗2天、4天、7天和14天的组（每个EGF治疗组n = 6，除14天组n = 4外）。对照组注射生理盐水（每组n = 3）。将切除的大肠细分为四个部分：升结肠、横结肠、降结肠和直肠。测量每个部分的长度和重量。通过径向切割大肠环获得零应力状态，并在视频图像上测量开口角度。测量黏膜层和肌层的厚度、横截面积以及内外周长。大肠总长度未增加，而大肠重量、黏膜厚度和质量以及内外周长显著增加（P < 0.05）。EGF治疗7天后，近端结肠的增加最为显著。此后，除大多数节段的黏膜厚度和降结肠的黏膜横截面积减小外，未观察到进一步的形态学变化。在对照组和EGF治疗的第一周，开口角度约为100度。14天后，升结肠和横结肠的开口角度显著增加至约172度和135度（P < 0.05）。总之，本研究表明全身EGF治疗以时间依赖性方式导致大肠零应力状态的形态重塑。生长在升结肠和横结肠最为明显，主要涉及黏膜层。
