上皮生长因子全身治疗后回肠的生物力学特性
Biomechanical properties of ileum after systemic treatment with epithelial growth factor.
作者信息
Yang Jian, Zhao Jing-Bo, Zeng Yan-Jun, Gregersen Hans
机构信息
Biomedical Engineering Center, Beijing Polytechnic University, China.
出版信息
World J Gastroenterol. 2003 Oct;9(10):2278-83. doi: 10.3748/wjg.v9.i10.2278.
AIM
Systemic treatment with epidermal growth factor (EGF) leads to growth of all parts of the small intestine in normal functioning rats. In this study, we investigated the effect of this growth process on morphometric and biomechanical parameters of ileum.
METHODS
Rats were treated with EGF (150 microg.kg(-1)day(-1))or placebo via osmotic minipumps for 2, 4, 7, and 14 days. A segment of ileum was removed. The morphology at no-load state and zero-stress state was measured and passive biomechanical properties were assessed using a biaxial test machine (combined inflation and axial stretching).
RESULTS
The ileum weight increased after EGF administration. After 4 days' EGF treatment, the wall thickness was increased. Significantly smaller inner perimeters were seen in 4 day and 7 day EGF treatment groups. The opening angle and residual strain began to increase after 7 days' EGF treatment. Wall stiffness, evaluated from the stress-strain curves, showed a continuous decrease in circumferential direction during the first 7 days' EGF treatment. The longitudinal stiffness increased during the first 7 days. The stress-strain curves for both circumferential and longitudinal direction tended to shift back to normal 14 days after starting EGF administration.
CONCLUSION
EGF can cause significant changes both in the morphology and in the passive mechanical properties of the rat ileum.
目的
用表皮生长因子（EGF）进行全身治疗可使功能正常的大鼠小肠各部分生长。在本研究中，我们调查了这一生长过程对回肠形态测量和生物力学参数的影响。
方法
通过渗透微型泵给大鼠注射EGF（150微克·千克⁻¹·天⁻¹）或安慰剂，持续2、4、7和14天。切除一段回肠。测量空载状态和零应力状态下的形态，并使用双轴试验机（联合充气和轴向拉伸）评估被动生物力学特性。
结果
给予EGF后回肠重量增加。EGF治疗4天后，肠壁厚度增加。在4天和7天的EGF治疗组中，内周长明显更小。EGF治疗7天后，开口角度和残余应变开始增加。从应力应变曲线评估的肠壁刚度在EGF治疗的前7天期间在圆周方向上持续下降。纵向刚度在最初7天增加。在开始给予EGF 14天后，圆周和纵向方向的应力应变曲线都趋于恢复正常。
结论
EGF可导致大鼠回肠的形态和被动力学特性发生显著变化。
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