• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从新分离的 6-氯烟碱矿化慢生根瘤菌菌株 SG-6C 中克隆新型 6-氯烟碱氯水解酶。

Cloning of a novel 6-chloronicotinic acid chlorohydrolase from the newly isolated 6-chloronicotinic acid mineralizing Bradyrhizobiaceae strain SG-6C.

机构信息

CSIRO Ecosystem Sciences, Australian Capital Territory, Australia.

出版信息

PLoS One. 2012;7(11):e51162. doi: 10.1371/journal.pone.0051162. Epub 2012 Nov 30.

DOI:10.1371/journal.pone.0051162
PMID:23226482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3511419/
Abstract

A 6-chloronicotinic acid mineralizing bacterium was isolated from enrichment cultures originating from imidacloprid-contaminated soil samples. This Bradyrhizobiaceae, designated strain SG-6C, hydrolytically dechlorinated 6-chloronicotinic acid to 6-hydroxynicotinic acid, which was then further metabolised via the nicotinic acid pathway. This metabolic pathway was confirmed by growth and resting cell assays using HPLC and LC-MS studies. A candidate for the gene encoding the initial dechlorination step, named cch2 (for 6-chloronicotinic acid chlorohydrolase), was identified using genome sequencing and its function was confirmed using resting cell assays on E. coli heterologously expressing this gene. The 464 amino acid enzyme was found to be a member of the metal dependent hydrolase superfamily with similarities to the TRZ/ATZ family of chlorohydrolases. We also provide evidence that cch2 was mobilized into this bacterium by an Integrative and Conjugative Element (ICE) that feeds 6-hydroxynicotinic acid into the existing nicotinic acid mineralization pathway.

摘要

从含有吡虫啉污染土壤样本的富集培养物中分离出一株 6-氯烟碱矿化菌。该Bradyrhizobiaceae 菌株命名为 SG-6C，可水解 6-氯烟碱生成 6-羟基烟碱，然后通过烟酸途径进一步代谢。通过使用 HPLC 和 LC-MS 研究的生长和静止细胞测定证实了这种代谢途径。使用基因组测序鉴定了编码初始脱氯步骤的候选基因 cch2（用于 6-氯烟碱氯水解酶），并通过在异源表达该基因的大肠杆菌静止细胞测定中证实了其功能。发现该 464 个氨基酸的酶是金属依赖水解酶超家族的成员，与 TRZ/ATZ 家族的氯水解酶具有相似性。我们还提供了证据表明，cch2 是由整合和共轭元件（ICE）转移到该细菌中的，该元件将 6-羟基烟碱输送到现有的烟酸矿化途径中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/8a454826b91c/pone.0051162.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/78f6c31e68fa/pone.0051162.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/5769bf2b0a6b/pone.0051162.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/b3d6820280fb/pone.0051162.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/1cc993139565/pone.0051162.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/8a454826b91c/pone.0051162.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/78f6c31e68fa/pone.0051162.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/5769bf2b0a6b/pone.0051162.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/b3d6820280fb/pone.0051162.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/1cc993139565/pone.0051162.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a316/3511419/8a454826b91c/pone.0051162.g005.jpg

相似文献

1
Cloning of a novel 6-chloronicotinic acid chlorohydrolase from the newly isolated 6-chloronicotinic acid mineralizing Bradyrhizobiaceae strain SG-6C.从新分离的 6-氯烟碱矿化慢生根瘤菌菌株 SG-6C 中克隆新型 6-氯烟碱氯水解酶。
PLoS One. 2012;7(11):e51162. doi: 10.1371/journal.pone.0051162. Epub 2012 Nov 30.
2
Genome sequence of the newly isolated chemolithoautotrophic Bradyrhizobiaceae strain SG-6C.新分离的化能自养慢生根瘤菌 SG-6C 菌株的基因组序列。
J Bacteriol. 2011 Sep;193(18):5057. doi: 10.1128/JB.05647-11. Epub 2011 Jul 8.
3
Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated Nocardioides sp. strain SG-4G and its potential for use in enzymatic bioremediation.新型诺卡氏菌 SG-4G 中咪鲜胺（甲基-1H-苯并咪唑-2-基氨基甲酸酯）水解酶的克隆和生化特性及其在酶法生物修复中的应用潜力。
Appl Environ Microbiol. 2010 May;76(9):2940-5. doi: 10.1128/AEM.02990-09. Epub 2010 Mar 12.
4
Isolation of new 6-methylnicotinic-acid-degrading bacteria, one of which catalyses the regioselective hydroxylation of nicotinic acid at position C2.新型6-甲基烟酸降解菌的分离，其中一种可催化烟酸在C2位的区域选择性羟基化反应。
Arch Microbiol. 1997 Nov;168(5):355-61. doi: 10.1007/s002030050509.
5
Column switching liquid chromatography and post-column photochemically fluorescence detection to determine imidacloprid and 6-chloronicotinic acid in honeybees.柱切换液相色谱法和柱后光化学荧光检测法测定蜜蜂体内的吡虫啉和6-氯烟酸。
J Chromatogr A. 2007 Apr 13;1147(1):17-23. doi: 10.1016/j.chroma.2007.02.015. Epub 2007 Feb 12.
6
Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazine.来自假单胞菌属菌株ADP的参与莠去津脱氯作用的一个基因区域的克隆、特性分析及表达
Appl Environ Microbiol. 1995 Sep;61(9):3373-8. doi: 10.1128/aem.61.9.3373-3378.1995.
7
Comparative genomic analysis of isoproturon-mineralizing sphingomonads reveals the isoproturon catabolic mechanism.异噁草松矿化鞘氨醇单胞菌的比较基因组分析揭示了异噁草松的代谢机制。
Environ Microbiol. 2016 Dec;18(12):4888-4906. doi: 10.1111/1462-2920.13413. Epub 2016 Jul 15.
8
A gene encoding a membrane protein exists upstream of the amoA/amoB genes in ammonia oxidizing bacteria: a third member of the amo operon?在氨氧化细菌中，编码膜蛋白的基因存在于amoA/amoB基因的上游：amo操纵子的第三个成员？
FEMS Microbiol Lett. 1997 May 1;150(1):65-73. doi: 10.1111/j.1574-6968.1997.tb10351.x.
9
Biological assessment of neonicotinoids imidacloprid and its major metabolites for potentially human health using globular proteins as a model.利用球蛋白作为模型对新烟碱类农药吡虫啉及其主要代谢物进行潜在人类健康的生物学评估。
J Photochem Photobiol B. 2015 Jun;147:24-36. doi: 10.1016/j.jphotobiol.2015.03.010. Epub 2015 Mar 23.
10
Heavy metal tolerant Metalliresistens boonkerdii gen. nov., sp. nov., a new genus in the family Bradyrhizobiaceae isolated from soil in Thailand.耐重金属的 Metalliresistens boonkerdii 属，新种，是从泰国土壤中分离出的布克氏菌科的一个新属。
Syst Appl Microbiol. 2010 Nov;33(7):374-82. doi: 10.1016/j.syapm.2010.06.002. Epub 2010 Jul 21.

引用本文的文献

1
Insights into the ubiquity, persistence and microbial intervention of imidacloprid.了解吡虫啉的普遍性、持久性和微生物干预。
Arch Microbiol. 2023 May 2;205(5):215. doi: 10.1007/s00203-023-03516-w.
2
Insights into the Toxicity and Degradation Mechanisms of Imidacloprid Via Physicochemical and Microbial Approaches.通过物理化学和微生物方法深入了解吡虫啉的毒性和降解机制
Toxics. 2020 Sep 1;8(3):65. doi: 10.3390/toxics8030065.
3
Insights Into the Microbial Degradation and Biochemical Mechanisms of Neonicotinoids.新烟碱类化合物的微生物降解及生化机制研究进展

本文引用的文献

1
Genomic organization and genomic structural rearrangements of Sphingobium japonicum UT26, an archetypal γ-hexachlorocyclohexane-degrading bacterium.苏云金芽胞杆菌 UT26 的基因组组织和基因组结构重排，这是一种典型的γ-六氯环己烷降解细菌。
Enzyme Microb Technol. 2011 Dec 10;49(6-7):499-508. doi: 10.1016/j.enzmictec.2011.10.005. Epub 2011 Nov 7.
2
The repertoire of ICE in prokaryotes underscores the unity, diversity, and ubiquity of conjugation.原核生物中 ICE 的 repertoire 凸显了接合的统一性、多样性和普遍性。
PLoS Genet. 2011 Aug;7(8):e1002222. doi: 10.1371/journal.pgen.1002222. Epub 2011 Aug 18.
3
Genome sequence of the newly isolated chemolithoautotrophic Bradyrhizobiaceae strain SG-6C.
Front Microbiol. 2020 May 19;11:868. doi: 10.3389/fmicb.2020.00868. eCollection 2020.
4
Insights into Ongoing Evolution of the Hexachlorocyclohexane Catabolic Pathway from Comparative Genomics of Ten Sphingomonadaceae Strains.通过对十株鞘脂单胞菌科菌株的比较基因组学研究洞察六氯环己烷分解代谢途径的持续进化
G3 (Bethesda). 2015 Apr 7;5(6):1081-94. doi: 10.1534/g3.114.015933.
5
Aerobic degradation of N-methyl-4-nitroaniline (MNA) by Pseudomonas sp. strain FK357 isolated from soil.土壤中分离出的假单胞菌 FK357 对 N-甲基-4-硝基苯胺（MNA）的好氧降解。
PLoS One. 2013 Oct 8;8(10):e75046. doi: 10.1371/journal.pone.0075046. eCollection 2013.
6
Biodegradation of the allelopathic chemical m-tyrosine by Bacillus aquimaris SSC5 involves the homogentisate central pathway.海栖芽孢杆菌SSC5对化感化学物质间酪氨酸的生物降解涉及尿黑酸中心途径。
PLoS One. 2013 Oct 1;8(10):e75928. doi: 10.1371/journal.pone.0075928. eCollection 2013.
7
Metabolism of 2-chloro-4-nitroaniline via novel aerobic degradation pathway by Rhodococcus sp. strain MB-P1.利用新型好氧降解途径，通过 Rhodococcus sp. 菌株 MB-P1 代谢 2-氯-4-硝基苯胺。
PLoS One. 2013 Apr 17;8(4):e62178. doi: 10.1371/journal.pone.0062178. Print 2013.
新分离的化能自养慢生根瘤菌 SG-6C 菌株的基因组序列。
J Bacteriol. 2011 Sep;193(18):5057. doi: 10.1128/JB.05647-11. Epub 2011 Jul 8.
4
Neonicotinoid insecticides: highlights of a symposium on strategic molecular designs.新烟碱类杀虫剂：策略性分子设计研讨会要点。
J Agric Food Chem. 2011 Apr 13;59(7):2883-6. doi: 10.1021/jf103856c. Epub 2010 Nov 15.
5
Ecotoxicity of neonicotinoid insecticides to bees.新烟碱类杀虫剂对蜜蜂的生态毒性。
Adv Exp Med Biol. 2010;683:85-95. doi: 10.1007/978-1-4419-6445-8_8.
6
Neonicotinoid metabolism: compounds, substituents, pathways, enzymes, organisms, and relevance.新烟碱类代谢：化合物、取代基、途径、酶、生物体及相关性。
J Agric Food Chem. 2011 Apr 13;59(7):2923-31. doi: 10.1021/jf102438c. Epub 2010 Aug 23.
7
Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow.整合子和转座子：镶嵌可移动遗传元件，实现动态水平基因转移。
Nat Rev Microbiol. 2010 Aug;8(8):552-63. doi: 10.1038/nrmicro2382. Epub 2010 Jul 5.
8
Cloning and biochemical characterization of a novel carbendazim (methyl-1H-benzimidazol-2-ylcarbamate)-hydrolyzing esterase from the newly isolated Nocardioides sp. strain SG-4G and its potential for use in enzymatic bioremediation.新型诺卡氏菌 SG-4G 中咪鲜胺（甲基-1H-苯并咪唑-2-基氨基甲酸酯）水解酶的克隆和生化特性及其在酶法生物修复中的应用潜力。
Appl Environ Microbiol. 2010 May;76(9):2940-5. doi: 10.1128/AEM.02990-09. Epub 2010 Mar 12.
9
Biotransformation of the neonicotinoid insecticides imidacloprid and thiamethoxam by Pseudomonas sp. 1G.假单胞菌1G对新烟碱类杀虫剂吡虫啉和噻虫嗪的生物转化
Biochem Biophys Res Commun. 2009 Mar 13;380(3):710-4. doi: 10.1016/j.bbrc.2009.01.156. Epub 2009 Jan 29.
10
Molecular recognition of neonicotinoid insecticides: the determinants of life or death.新烟碱类杀虫剂的分子识别：生死的决定因素
Acc Chem Res. 2009 Feb 17;42(2):260-9. doi: 10.1021/ar800131p.