球形链霉菌1912和产氰链霉菌S136中landomycins的产生受假定转录激活因子编码基因的调控。

Production of landomycins in Streptomyces globisporus 1912 and S cyanogenus S136 is regulated by genes encoding putative transcriptional activators.

作者信息

Rebets Y, Ostash B, Luzhetskyy A, Hoffmeister D, Brana A, Mendez C, Salas J A, Bechthold A, Fedorenko V

机构信息

Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskyy st 4, L'viv 79005, Ukraine.

出版信息

FEMS Microbiol Lett. 2003 May 16;222(1):149-53. doi: 10.1016/S0378-1097(03)00258-1.

DOI:10.1016/S0378-1097(03)00258-1

PMID:12757959

Abstract

The regulatory genes lanI and lndI have been cloned from the landomycin A (LaA) producer Streptomyces cyanogenus S136 and from the landomycin E (LaE) producer Streptomyces globisporus 1912, respectively and both have been sequenced. A culture of S. globisporus I2-1 carrying a disrupted lndI gene did not produce LaE and other related intermediates. Complementation of S. globisporus I2-1 with either the lndI or lanI gene reconstituted LaE production indicating that LanI and LndI are involved in activation of structural genes in the respective clusters. Structural features of these regulatory genes are discussed.

摘要

调控基因lanI和lndI分别从产土霉素A（LaA）的蓝青色链霉菌S136和产土霉素E（LaE）的球形孢链霉菌1912中克隆得到，且二者均已测序。携带lndI基因被破坏的球形孢链霉菌I2-1培养物不产生LaE及其他相关中间体。用lndI或lanI基因对球形孢链霉菌I2-1进行互补，可恢复LaE的产生，这表明LanI和LndI参与了各自基因簇中结构基因的激活。文中讨论了这些调控基因的结构特征。