Myronovskyi Maksym, Rosenkränzer Birgit, Stierhof Marc, Petzke Lutz, Seiser Tobias, Luzhetskyy Andriy
Pharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, Germany.
BASF SE, 67056 Ludwigshafen, Germany.
Microorganisms. 2020 Feb 10;8(2):237. doi: 10.3390/microorganisms8020237.
Herbicides with new modes of action and safer toxicological and environmental profiles are needed to manage the evolution of weeds that are resistant to commercial herbicides. The unparalleled structural diversity of natural products makes these compounds a promising source for new herbicides. In 2009, a novel nucleoside phytotoxin, albucidin, with broad activity against grass and broadleaf weeds was isolated from a strain of subsp. NRRL B-24108. Here, we report the identification and heterologous expression of the previously uncharacterized albucidin gene cluster. Through a series of gene inactivation experiments, a minimal set of albucidin biosynthetic genes was determined. Based on gene annotation and sequence homology, a model for albucidin biosynthesis was suggested. The presented results enable the construction of producer strains for a sustainable supply of albucidin for biological activity studies.
需要具有新作用模式以及更安全毒理学和环境特征的除草剂来应对对商业除草剂产生抗性的杂草的进化。天然产物无与伦比的结构多样性使这些化合物成为新型除草剂的一个有前景的来源。2009年，从一种 亚种菌株中分离出一种对禾本科和阔叶杂草具有广泛活性的新型核苷植物毒素——杀草啶。在此，我们报告了此前未被表征的杀草啶基因簇的鉴定和异源表达。通过一系列基因失活实验，确定了杀草啶生物合成基因的最小集合。基于基因注释和序列同源性，提出了杀草啶生物合成的模型。所呈现的结果有助于构建生产菌株，以可持续地供应杀草啶用于生物活性研究。
