胆碱鏻类似物在培养细胞中的代谢。一种用于膜磷脂酰胆碱的有用核磁共振探针。
The metabolism of the phosphonium analogue of choline in cultured cells. A useful nuclear-magnetic-resonance probe for membrane phosphatidylcholine.
作者信息
Sim E, Pasternak C A
出版信息
Biochem J. 1976 Jan 15;154(1):105-11. doi: 10.1042/bj1540105.
Abstract
- Replacement of choline by the phosphonium analogue does not affect the growth rate of P815Y, NIL, 3T3, and SV40/3T3 cells in culture. 2. The fatty acid composition of the resulting phosphonium phosphatidylcholine is similar to that of phosphatidylcholine. 3. The rate of synthesis and degradation of phosphatidylcholine and of the phosphonium analogue are similar. 4. Phospholipid-exchange protein does not distinguish between phosphatidylcholine and the phosphonium analogue. 5. By contrast, incorporation of phosphonium choline into sphingomyelin occurs to only a minor extent. 6. It is concluded that, since the enzymes involved in the turnover of phosphatidylcholine do not discriminate between quaternary N and quaternary P in the polar head-group region, phosphonium choline should prove to be a useful probe for 31P nuclear-magnetic-resonance (n.m.r.) studies of natural membranes.
摘要
- 用鏻类似物取代胆碱不会影响培养的P815Y、NIL、3T3和SV40/3T3细胞的生长速率。2. 所得鏻磷脂酰胆碱的脂肪酸组成与磷脂酰胆碱相似。3. 磷脂酰胆碱和鏻类似物的合成与降解速率相似。4. 磷脂交换蛋白无法区分磷脂酰胆碱和鏻类似物。5. 相比之下，鏻胆碱掺入鞘磷脂的程度很小。6. 得出的结论是，由于参与磷脂酰胆碱周转的酶在极性头部区域不会区分季铵氮和季铵磷，因此鏻胆碱应被证明是用于天然膜的31P核磁共振（n.m.r.）研究的有用探针。
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