哺乳动物磷脂的周转。肿瘤性肥大细胞中的稳定和不稳定成分。
Turnover of mammalian phospholipids. Stable and unstable components in neoplastic mast cells.
作者信息
Pasternak C A, Bergeron J J
出版信息
Biochem J. 1970 Sep;119(3):473-80. doi: 10.1042/bj1190473.
Abstract
- Choline- and inositol-labelled phospholipids of exponentially growing or static neoplastic mast cells turn over by degradation and resynthesis of the entire molecule. Turnover follows a biphasic pattern, the unstable rapidly turning-over component accounting for 60-80% of labelled phospholipid. The residual stable component does not turn over any more than does protein or DNA. 2. Subcellular fractions and surface membranes of choline-labelled P815Y cells contain the same proportion of stable and unstable components as do whole cells. The unstable component is largely phosphatidylcholine; the stable component is relatively richer in sphingomyelin. 3. It is concluded that the phospholipids of neoplastic mast cells are of two classes, one of which is susceptible to continual enzymic degradation and resynthesis, and the other of which is metabolically stable.
摘要
- 处于指数生长期或静止期的肿瘤性肥大细胞中，胆碱和肌醇标记的磷脂通过整个分子的降解和再合成进行周转。周转呈双相模式，不稳定的快速周转成分占标记磷脂的60 - 80%。剩余的稳定成分的周转情况与蛋白质或DNA无异。2. 胆碱标记的P815Y细胞的亚细胞组分和表面膜中，稳定和不稳定成分的比例与全细胞相同。不稳定成分主要是磷脂酰胆碱；稳定成分中鞘磷脂相对更丰富。3. 得出的结论是，肿瘤性肥大细胞的磷脂分为两类，其中一类易受持续的酶促降解和再合成影响，另一类在代谢上是稳定的。
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The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. Phospholipid and macromolecular synthesis in neoplastic mast cells.利用常规离心法和区带离心法研究哺乳动物细胞的生命周期。肿瘤性肥大细胞中的磷脂和大分子合成。
Biochem J. 1970 Sep;119(3):493-9. doi: 10.1042/bj1190493.
10
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Biochem J. 1970 Sep;119(3):489-92. doi: 10.1042/bj1190489.
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