Sardo Pierangelo, Ferraro Giuseppe, Di Giovanni Giuseppe, La Grutta Vittorio
Dipartimento di Medicina Sperimentale--Sezione di Fisiologia umana, Università degli Studi di Palermo, Corso Tukory, 129-90134 Palermo, Italy.
Neurosci Lett. 2003 Jun 5;343(2):101-4. doi: 10.1016/s0304-3940(03)00350-1.
Single units were recorded in the striatum and in the globus pallidus (GP) of urethane-anesthetized rats under microiontophoretic administration of either Nomega-nitro-L-arginine methyl ester (L-NAME, inhibitor of nitric oxide synthase), or 3-morpholino-sydnonimin-hydrocloride (SIN-1, nitric oxide, NO donor). A steady baseline firing of sporadically discharging striatal neurons (basal firing rate <0.1 spikes/s) was evoked by a pulsed microiontophoretic ejection of glutamate. On striatal neurons, microiontophoretic application of SIN-1 induced a current-dependent inhibition (11/13), whereas L-NAME administration produced a clear excitation (9/9). On GP cells, the administration of SIN-1 had excitatory effects (10/15), whereas the administration of L-NAME reduced the neuronal activity (6/6). We hypothesize that NO could exert an intrinsic regulatory action on the activity of both striatal and GP cells.
在氨基甲酸乙酯麻醉的大鼠纹状体和苍白球（GP）中，在微量离子电泳给予N-甲基-L-精氨酸甲酯（L-NAME，一氧化氮合酶抑制剂）或3-吗啉代-西多芬宁盐酸盐（SIN-1，一氧化氮供体）的情况下记录单个神经元活动。通过谷氨酸的脉冲微量离子电泳喷射诱发了散在放电的纹状体神经元稳定的基线放电（基础放电率<0.1个脉冲/秒）。在纹状体神经元上，微量离子电泳应用SIN-1诱导了电流依赖性抑制（11/13），而给予L-NAME则产生了明显的兴奋作用（9/9）。在GP细胞上，给予SIN-1有兴奋作用（10/15），而给予L-NAME则降低了神经元活动（6/6）。我们推测一氧化氮可能对纹状体和GP细胞的活动发挥内在调节作用。
