Williams Kristi L, Taxman Debra J, Linhoff Michael W, Reed William, Ting Jenny P-Y
Department of Microbiology-Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.
J Immunol. 2003 Jun 1;170(11):5354-8. doi: 10.4049/jimmunol.170.11.5354.
Proteins containing a limited number of N-terminal motifs followed by nucleotide-binding domain and leucine-rich repeat regions are emerging as important regulators for immunity. A search of human genome scaffold databases has identified a large family of known and unknown genes, which we have recently called the CATERPILLER (caspase recruitment domain, transcription enhancer, r(purine)-binding, pyrin, lots of leucine repeats) gene family. This work describes the characterization of a new member, Monarch-1. Monarch-1 has four different splice forms due to the differential splicing of leucine-rich repeat motifs. It is expressed in cells of myeloid-monocytic origin. Affymetrix microarrays and small interfering RNA were used to elucidate the downstream effects of Monarch-1 expression in cells including those of myeloid-monocytic origin. These analyses show that Monarch-1 enhances nonclassical and classical MHC class I expression at the level of the promoter, RNA, and protein expression.
含有有限数量的N端基序，随后是核苷酸结合结构域和富含亮氨酸重复区域的蛋白质正逐渐成为免疫的重要调节因子。对人类基因组支架数据库的搜索已鉴定出一个由已知和未知基因组成的大家族，我们最近将其称为CATERPILLER（半胱天冬酶招募结构域、转录增强子、r（嘌呤）结合、吡啉、大量亮氨酸重复）基因家族。这项工作描述了一个新成员Monarch-1的特征。由于富含亮氨酸重复基序的差异剪接，Monarch-1有四种不同的剪接形式。它在髓系单核细胞来源的细胞中表达。使用Affymetrix微阵列和小干扰RNA来阐明Monarch-1在包括髓系单核细胞来源的细胞中的表达的下游效应。这些分析表明，Monarch-1在启动子、RNA和蛋白质表达水平上增强非经典和经典MHC I类表达。
