Casella Innocenzo G, Contursi Michela
Dipartimento di Chimica, Universita' degli Studi della Basilicata, Via N. Sauro 85, 85100, Potenza, Italy.
Anal Bioanal Chem. 2003 Jul;376(5):673-9. doi: 10.1007/s00216-003-1948-4. Epub 2003 May 21.
A cobalt oxyhydroxide film dispersed on a carbon electrode surface was characterized and proposed as an amperometric sensor for determination of alditols and carbohydrates in flowing streams. Complex mixtures of carbohydrates were separated by anion-exchange chromatography using a moderately alkaline solution as mobile phase. The cobalt modified electrode (GC-Co) was employed under a constant applied potential of 0.5 V (vs Ag/AgCl). Under these experimental conditions the detection limits (S/N=3) for all analyzed electroactive molecules ranged between 0.3 micromol L(-1) and 1.5 micromol L(-1) and the dynamic linear ranges spanned generally three orders of magnitude above the relevant detection limits. Analytical determinations of carbohydrates and alditols in red and white wines, are reported.
对分散在碳电极表面的氢氧化氧钴薄膜进行了表征，并提出将其作为安培传感器用于测定流动样品中的糖醇和碳水化合物。使用中等碱性溶液作为流动相，通过阴离子交换色谱法分离碳水化合物的复杂混合物。钴修饰电极（玻碳-钴电极，GC-Co）在0.5 V（相对于Ag/AgCl）的恒定外加电位下使用。在这些实验条件下，所有分析的电活性分子的检测限（信噪比S/N = 3）在0.3 μmol L⁻¹至1.5 μmol L⁻¹之间，动态线性范围通常在相关检测限以上跨越三个数量级。报告了红葡萄酒和白葡萄酒中碳水化合物和糖醇的分析测定结果。
