[应用聚合酶链反应和原位杂交技术检测慢性乙型肝炎及肝细胞癌患者外周血单个核细胞中的TT病毒DNA]

[Detection of TT virus DNA in peripheral blood mononuclear cells in patients with chronic hepatitis B and hepatocellular carcinoma using PCR and in situ hybridization].

作者信息

Liu Z, Zhou Y, Dong J F

机构信息

Beijing Post and Telecommunication General Hospital, Beijing 100032.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1999 Dec;13(4):332-4.

PMID:12759970

Abstract

OBJECTIVE

To determine whether the TTV infection occurs in peripheral blood mononuclear cells (PBMCs) of patients with chronic hepatitis B (of medium degree) and in HBsAg positive hepatocellular carcinoma (HCC).

METHODS

TTV DNA in PBMCs was detected by nested-polymerase chain reaction(PCR) and in situ hybridization (ISH).

RESULTS

7 of 26 cases of chronic hepatitis B were positive for TTV DNA by nested-PCR with a positive rate of 26.9% that was significantly higher than that of normal control(chi 2 = 14.3, P < 0.001); 4 of 21 HBsAg positive cases were positive for TTV DNA by nested-PCR with a positive rate of 19.0%, also significantly higher than that of normal control(chi 2 = 4.86, P < 0.05). 4 of 7 TTV positive patients with chronic hepatitis B tested by nested-PCR were positive for TTV DNA by ISH in PBMC cytoplasm, with a positive rate of 57.1%.

CONCLUSION

(1) A higher incidence of TTV DNA in PBMCs of patients with chronic hepatitis B and in HBsAg positive HCC was identified by nested-PCR and ISH; (2) TTV DNA was in the cytoplasm of PBMCs in patients with chronic hepatitis B.

摘要

目的

确定TTV感染是否发生于慢性乙型肝炎（中度）患者的外周血单个核细胞（PBMC）以及HBsAg阳性的肝细胞癌（HCC）患者中。

方法

采用巢式聚合酶链反应（PCR）和原位杂交（ISH）检测PBMC中的TTV DNA。

结果

26例慢性乙型肝炎患者中，7例巢式PCR检测TTV DNA呈阳性，阳性率为26.9%，显著高于正常对照组（χ2 = 14.3，P < 0.001）；21例HBsAg阳性患者中，4例巢式PCR检测TTV DNA呈阳性，阳性率为19.0%，也显著高于正常对照组（χ2 = 4.86，P < 0.05）。7例巢式PCR检测TTV阳性的慢性乙型肝炎患者中，4例PBMC细胞质ISH检测TTV DNA呈阳性，阳性率为57.1%。