Zhu F, Qian J M, Yang X O, Sun G, Lu C M
Department of Gastroenterology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2001 Dec;23(6):627-30.
To clarify the relationship between TT virus (TTV) infection and the development of hepatocellular carcinoma (HCC).
TTV was examined in sera of 112 patients with HCC and 95 patients with chronic liver disease (CLD) without HCC. 30 patients without liver disease were examined as control. TTV DNA was detected by the second generation of TTV PCR primers (NG059, NG061 and NG063) after extraction of DNA from 50 microliters serum. The relationship between TTV infection and clinical features of each group patients was analyzed.
TTV DNA was detected in 17.86% (20/112) of HCC patients, and this prevalence in HCC patients was not significantly higher than that of TTV DNA in CLD patients (15.78%, 15/95). There were no significant differences in age, gender, tumor factors (size, TNM classification and markers) and other hepatitis virus markers between TTV-positive and TTV-negative in HCC and CLD patients, except history of blood transfusion [(80.00% vs 35.87% (P < 0.05) in HCC patients and 73.33% vs 27.50% (P < 0.05) in CLD patients)] and ALT [(93 +/- 27) IU/L vs (68 +/- 31) IU/L (P < 0.05) in HCC patients]. It seemed that TTV did not do any harm to the liver function of patients who infected with HBV or HCV, except for elevating ALT level in such a kind of HCC patients.
Although TTV could do harm to the liver function of HCC patients, it does not contribute to the development of HCC from CLD.
阐明TTV病毒（TTV）感染与肝细胞癌（HCC）发生发展之间的关系。
检测112例肝细胞癌患者和95例无肝细胞癌的慢性肝病（CLD）患者血清中的TTV。选取30例无肝病患者作为对照。从50微升血清中提取DNA后，采用第二代TTV PCR引物（NG059、NG061和NG063）检测TTV DNA。分析TTV感染与各组患者临床特征之间的关系。
肝细胞癌患者中17.86%（20/112）检测到TTV DNA，其在肝细胞癌患者中的流行率并不显著高于慢性肝病患者（15.78%，15/95）。肝细胞癌和慢性肝病患者中，TTV阳性和TTV阴性患者在年龄、性别、肿瘤因素（大小、TNM分期和标志物）以及其他肝炎病毒标志物方面均无显著差异，但输血史存在差异[肝细胞癌患者中分别为80.00%和35.87%（P<0.05），慢性肝病患者中分别为73.33%和27.50%（P<0.05）]，谷丙转氨酶（ALT）也存在差异[肝细胞癌患者中分别为（93±27）IU/L和（68±31）IU/L（P<0.05）]。似乎TTV对感染乙肝病毒或丙肝病毒的患者肝功能无损害，只是会使此类肝细胞癌患者的ALT水平升高。
虽然TTV会损害肝细胞癌患者的肝功能，但它不会促使慢性肝病发展为肝细胞癌。
