Skubitz Keith M, Skubitz Amy P N
Department of Medicine, University of Minnesota Medical School, Minneapolis, USA.
J Lab Clin Med. 2003 May;141(5):297-308. doi: 10.1016/S0022-2143(03)00007-6.
Uterine leiomyomas are the most common tumor of the genitourinary system in women and are a major cause of morbidity. The molecular causes of the disease remain unclear. In this study, we examined gene expression in leiomyomas and normal myometrium. RNA was prepared and gene expression determined with the use of Affymetrix GeneChip U_95 arrays containing approximately 12,000 known genes and 48,000 expression sequence tags. Several genes were found to be differentially expressed in these two sample sets, and these genes were analyzed for their expression in a variety of other normal and diseased tissues. Four genes--doublecortin, calpain 6, interleukin-17B, and proteolipid protein 1--were found to be overexpressed in leiomyomas compared with normal myometrium and eighteen other tissues. Sets of genes were identified whose expression could be used to cluster samples with leiomyomas or normal myometrium with the use of Eisen Cluster software. We conclude that differences in gene expression can be detected between leiomyomas and normal myometrium and that these changes in gene expression may yield clues to the pathophysiology of this common tumor.
子宫平滑肌瘤是女性生殖泌尿系统最常见的肿瘤，也是发病的主要原因。该疾病的分子病因尚不清楚。在本研究中，我们检测了平滑肌瘤和正常子宫肌层中的基因表达。制备了RNA，并使用包含约12,000个已知基因和48,000个表达序列标签的Affymetrix GeneChip U_95阵列测定基因表达。发现几个基因在这两个样本组中差异表达，并分析了这些基因在各种其他正常和患病组织中的表达。与正常子宫肌层和其他18种组织相比，发现双皮质素、钙蛋白酶6、白细胞介素-17B和蛋白脂质蛋白1这四个基因在平滑肌瘤中过表达。利用艾森聚类软件确定了一些基因集，其表达可用于将平滑肌瘤样本或正常子宫肌层样本聚类。我们得出结论，平滑肌瘤和正常子宫肌层之间可以检测到基因表达差异，并且这些基因表达变化可能为这种常见肿瘤的病理生理学提供线索。
