Li Tian, Li Xiao-Mao, Yin Heng-Hui, Yang Yue-Bo
Department of Gynecology and Obstetrics, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510630, PR China.
Ai Zheng. 2004 Mar;23(3):292-5.
BACKGROUND & OBJECTIVE: The occurrence of uterine leiomyoma is related to the abnormal expression of some genes, but the molecular mechanism has not been clarified yet, the study screened genes related to uterine leiomyoma by comparing different genes expressed in uterine leiomyoma tissues with normal myometrium uterine tissues.
To compare different genes expressed in uterine leiomyoma tissues with normal myometrium uterine tissues by fluorescent differential display reverse transcription polymerase chain reaction (FDD-RTPCR). Four sets of anchor primers and three sets of arbitrary primers, totally twelve primer combinations, were used for FDD-RTPCR amplification. Differentially expressed cDNA fragments were cloned, sequenced, and analyzed in origin. And one of differentially expressed bands was analyzed by RT-PCR.
Twenty-seven cDNA fragments were isolated from uterine leiomyoma. N(568) cDNA fragment shared 96% homology with the sequence of GCRG114 gene. RT-PCR confirmed that this gene was expressed lowlier in uterine leiomyoma tissues than in normal myometrium uterine tissues.
N(568) cDNA fragment is highly homologous with the sequence of GCRG114 gene, and it may be related to the development of uterine leiomyoma.
子宫肌瘤的发生与某些基因的异常表达有关，但其分子机制尚未阐明。本研究通过比较子宫肌瘤组织与正常子宫肌层组织中不同的基因表达情况，筛选出与子宫肌瘤相关的基因。
采用荧光差异显示逆转录聚合酶链反应（FDD-RTPCR）比较子宫肌瘤组织与正常子宫肌层组织中不同的基因表达。FDD-RTPCR扩增使用4套锚定引物和3套随机引物，共12种引物组合。对差异表达的cDNA片段进行克隆、测序及同源性分析，并对其中一条差异表达条带进行RT-PCR分析。
从子宫肌瘤组织中分离出27个cDNA片段。N(568) cDNA片段与GCRG114基因序列有96%的同源性。RT-PCR证实该基因在子宫肌瘤组织中的表达低于正常子宫肌层组织。
N(568) cDNA片段与GCRG114基因序列高度同源，可能与子宫肌瘤的发生发展有关。
