Bleich H E, Cutnell J D, Glasel J A
Biochemistry. 1976 Jun 1;15(11):2455-66. doi: 10.1021/bi00656a032.
Measurements of 1H and 13C spin-lattice relaxation and 13C nuclear Overhauser enhancement factors are reported for dimethyl sulfoxide solutions of tetragastrin, a pharmacologically active tetrapeptide. The use of the dipolar formalism for predicting 1H and quaternary 13C relaxation rates is discussed. Furthermore, the prospect is opened for the use of quaternary 13C and 1H relaxation times to obtain information on the peptide torsion angles phi, psi, and chi in a way supplementing NMR coupling constant methods presently in use.
报道了胃泌素（一种具有药理活性的四肽）在二甲基亚砜溶液中的1H和13C自旋晶格弛豫以及13C核Overhauser增强因子的测量结果。讨论了使用偶极形式来预测1H和季碳13C弛豫速率。此外，开辟了利用季碳13C和1H弛豫时间来获取肽扭转角phi、psi和chi信息的前景，以补充目前使用的NMR耦合常数方法。
