Clark R W
Biochim Biophys Acta. 1976 Apr 23;428(2):269-74. doi: 10.1016/0304-4165(76)90034-9.
A mathematical method is described for calculating the sedimentation coefficient (S20,W) with ultracentrifuge data from linear sucrose gradients. Gradient density and viscosity functions are precisely described by regression equations, which permit continuous evaluation (by integration) of the effects of gradient geometry on particle sedimentation. The results agree with previously used and more complex methods.
描述了一种利用线性蔗糖梯度超速离心数据计算沉降系数（S20,W）的数学方法。梯度密度和粘度函数由回归方程精确描述，这允许通过积分连续评估梯度几何形状对颗粒沉降的影响。结果与先前使用的更复杂方法一致。
