来自丛毛单胞菌属SMN4菌株的2,3-二羟基联苯1,2-双加氧酶基因（bphC）的克隆及序列分析，用于系统发育和结构分析。
Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene ( bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis.
作者信息
Lee N R, Kwon D Y, Min K H
机构信息
Korea Food Research Institute, Baekhyon, Poondang, Songnam, 463-746, Kyongki-do, Korea.
出版信息
J Ind Microbiol Biotechnol. 2003 Apr;30(4):245-50. doi: 10.1007/s10295-003-0039-z. Epub 2003 Mar 20.
A genomic library of biphenyl-degrading Comamonas sp. SMN4 for isolating fragments containing the 2,3-dihydroxybiphenyl 1,2-dioxygenase (23DBDO) gene was constructed. The smallest subclone (pNPX9) encoding 23DBDO activity was sequenced and analyzed. The C-terminal domain of 23DBDO from Comamonas sp. SMN4 had five catalytically essential residues and was more highly conserved than the N-terminal domain. Phylogenetic and structural relationships of 23DBDO from Comamonas sp. SMN4 were analyzed.
构建了联苯降解丛毛单胞菌属菌株SMN4的基因组文库，用于分离含有2,3-二羟基联苯1,2-双加氧酶（23DBDO）基因的片段。对编码23DBDO活性的最小亚克隆（pNPX9）进行了测序和分析。丛毛单胞菌属菌株SMN4的23DBDO的C末端结构域有五个催化必需残基，并且比N末端结构域保守性更高。分析了丛毛单胞菌属菌株SMN4的23DBDO的系统发育和结构关系。
Zotero 插件