Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G
Institut de Genetique et de Biologie Moleculaire et Cellulaire (CNRS/INSERM/ULP), BP 163, 67404 Illkirch Cedex, France.
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82. doi: 10.1093/nar/25.24.4876.
CLUSTAL X is a new windows interface for the widely-used progressive multiple sequence alignment program CLUSTAL W. The new system is easy to use, providing an integrated system for performing multiple sequence and profile alignments and analysing the results. CLUSTAL X displays the sequence alignment in a window on the screen. A versatile sequence colouring scheme allows the user to highlight conserved features in the alignment. Pull-down menus provide all the options required for traditional multiple sequence and profile alignment. New features include: the ability to cut-and-paste sequences to change the order of the alignment, selection of a subset of the sequences to be realigned, and selection of a sub-range of the alignment to be realigned and inserted back into the original alignment. Alignment quality analysis can be performed and low-scoring segments or exceptional residues can be highlighted. Quality analysis and realignment of selected residue ranges provide the user with a powerful tool to improve and refine difficult alignments and to trap errors in input sequences. CLUSTAL X has been compiled on SUN Solaris, IRIX5.3 on Silicon Graphics, Digital UNIX on DECstations, Microsoft Windows (32 bit) for PCs, Linux ELF for x86 PCs, and Macintosh PowerMac.
CLUSTAL X是一款面向广泛使用的渐进式多序列比对程序CLUSTAL W的全新Windows界面。新系统易于使用，提供了一个用于执行多序列和轮廓比对以及分析结果的集成系统。CLUSTAL X在屏幕上的一个窗口中显示序列比对。一种通用的序列着色方案允许用户突出显示比对中的保守特征。下拉菜单提供了传统多序列和轮廓比对所需的所有选项。新功能包括：能够剪切和粘贴序列以更改比对顺序、选择要重新比对的序列子集，以及选择要重新比对并插入回原始比对的比对子范围。可以进行比对质量分析，并突出显示低分片段或异常残基。对选定残基范围进行质量分析和重新比对为用户提供了一个强大的工具，可用于改进和完善困难的比对，并发现输入序列中的错误。CLUSTAL X已在SUN Solaris、Silicon Graphics上的IRIX5.3、DECstations上的Digital UNIX、适用于个人计算机的Microsoft Windows（32位）、适用于x86个人计算机的Linux ELF以及Macintosh PowerMac上编译。
