Furukawa K, Simon J R, Chakrabarty A M
J Bacteriol. 1983 Jun;154(3):1356-62. doi: 10.1128/jb.154.3.1356-1362.1983.
A strain of Pseudomonas paucimobilis (strain Q1) capable of utilizing biphenyl was isolated from soil. This strain grew not only on substituted biphenyls, but also on salicylate, xylene or toluene or both (xylene/toluene), and substituted benzoates. Evidence is presented that the catabolism of biphenyl, xylene/toluene, and salicylate is regulated by a common unit in this strain. The catabolism of biphenyl, xylene/toluene, and salicylate is interrelated, since benzoate and toluate are common metabolic intermediates of biphenyl and xylene/toluene, and salicylate is produced from 2-hydroxybiphenyl (o-phenylphenol). All the oxidative enzymes of the biphenyl, xylene/toluene, and salicylate degradative pathways were induced when the cells were grown on either biphenyl, xylene/toluene or salicylate. The P. paucimobilis Q1 cells showed induction of the meta-cleavage enzymes of both 2,3-dihydroxybiphenyl and catechol. Biphenyl-negative derivatives of strain Q1 were simultaneously rendered xylene/toluene and salicylate negative, whereas reversion to the biphenyl-positive character of such derivatives invariably led to a xylene/toluene- and salicylate-positive phenotype. Growth of the P. paucimobilis Q1 cells with benzoate as a sole carbon source allowed the induction of only the ortho pathway enzymes, suggesting that biphenyl, xylene/toluene, or salicylate specifically induced the meta pathway enzymes for the oxidative degradation of these compounds.
从土壤中分离出了一株能够利用联苯的少动假单胞菌（菌株Q1）。该菌株不仅能在取代联苯上生长，还能在水杨酸盐、二甲苯或甲苯或两者（二甲苯/甲苯）以及取代苯甲酸盐上生长。有证据表明，该菌株中联苯、二甲苯/甲苯和水杨酸盐的分解代谢受一个共同单元调控。联苯、二甲苯/甲苯和水杨酸盐的分解代谢相互关联，因为苯甲酸盐和甲苯酸盐是联苯和二甲苯/甲苯的共同代谢中间体，水杨酸盐由2-羟基联苯（邻苯基苯酚）产生。当细胞在联苯、二甲苯/甲苯或水杨酸盐上生长时，联苯、二甲苯/甲苯和水杨酸盐降解途径的所有氧化酶都会被诱导。少动假单胞菌Q1细胞显示出2,3-二羟基联苯和儿茶酚的间位裂解酶的诱导。菌株Q1的联苯阴性衍生物同时变为二甲苯/甲苯和水杨酸盐阴性，而此类衍生物回复到联苯阳性特征时总是导致二甲苯/甲苯和水杨酸盐阳性表型。以苯甲酸盐作为唯一碳源培养少动假单胞菌Q1细胞仅能诱导邻位途径酶，这表明联苯、二甲苯/甲苯或水杨酸盐特异性地诱导了这些化合物氧化降解的间位途径酶。
