Bukowiecki L, Himms-Hagen J
Biochim Biophys Acta. 1976 May 28;428(3):591-9. doi: 10.1016/0304-4165(76)90187-2.
Incorporation of L-[U-14C] leucine into liver, brown adipose tissue and skeletal muscle mitochondrial proteins was determined in vivo and in vitro during cold-acclimation. Major alterations in mitochondrial protein metabolism were observed in brown adipose tissue and skeletal muscle but not in liver. Immediate cold-exposure is accompanied by an inhibition of the in vivo incorporation of L-[U-14C] leucine into mitochondrial proteins of all tissues. However, during cold-acclimation the incorporation of leucine increases markedly in brown adipose tissue, continues to decrease in skeletal muscle, nut does not change appreciably in the liver. Because increased incorporation of L-[U-14C]-leucine into brown adipose tissue mitochondrial proteins was observed both in vivo and in vitro, it can be concluded that the mitochondrial protein-synthesizing system of this tissue is directly affected by the acclimation process. The observed changes in mitochondrial protein metabolism of brown adipose tissue and skeletal muscle might be responsible for the development of several morphological and biochemical alterations that characterize the establishment in these tissues of the cold-acclimated state.
在冷适应过程中，对体内和体外L-[U-¹⁴C]亮氨酸掺入肝脏、棕色脂肪组织和骨骼肌线粒体蛋白的情况进行了测定。在棕色脂肪组织和骨骼肌中观察到线粒体蛋白代谢的主要变化，而肝脏中未观察到。立即暴露于寒冷环境会抑制体内L-[U-¹⁴C]亮氨酸掺入所有组织的线粒体蛋白。然而，在冷适应过程中，亮氨酸掺入在棕色脂肪组织中显著增加，在骨骼肌中持续减少，而在肝脏中没有明显变化。由于在体内和体外均观察到L-[U-¹⁴C]亮氨酸掺入棕色脂肪组织线粒体蛋白增加，因此可以得出结论，该组织的线粒体蛋白合成系统直接受到适应过程的影响。棕色脂肪组织和骨骼肌线粒体蛋白代谢的观察到的变化可能是这些组织中表征冷适应状态建立的几种形态学和生化改变发展的原因。
