Kobayashi Kohmei, Kamiie Katsuyoshi, Kobayashi Akihiro, Matsuda Satoru
Department of Nursing, The Japanese Red Cross Hiroshima College of Nursing, Hatsukaichi, Japan.
J Basic Microbiol. 2003;43(3):249-54. doi: 10.1002/jobm.200390028.
Mycobacterium bovis BCG has been used for the prevention of tuberculosis and as therapy for bladder tumor. MPB63 in M. bovis BCG is one of the immunogenic proteins and is secreted in large quantities. Therefore, it is of interest that the MPB63 gene be examined for the determination of its nucleotide sequence. A fragment of 820 base pairs (bp) including the MPB63 gene was prepared by amplification using polymerase chain reaction (PCR) employing M. bovis BCG Tokyo chromosomal DNA as a template and its nucleotide sequence was determined. The nucleotide sequence of mpb63 in M. bovis BCG was then compared with that of mpt63 in M. tuberculosis. The result indicated that the nucleotide sequences between two protein genes were quite agreeable in the genes' structural and upstream regions, except that one base change from C in mpt63 to G in mpb63 was detected in the downstream trailer sequence. This suggests that the genetic information of M. bovis BCG is not entirely identical to that of M. tuberculosis, although the characteristics of both microorganism are very similar to each other.
牛分枝杆菌卡介苗（Mycobacterium bovis BCG）已被用于预防结核病以及作为膀胱肿瘤的治疗手段。牛分枝杆菌卡介苗中的MPB63是一种免疫原性蛋白，且大量分泌。因此，对MPB63基因进行核苷酸序列测定很有意义。以牛分枝杆菌卡介苗东京株染色体DNA为模板，通过聚合酶链反应（PCR）扩增制备了一个包含MPB63基因的820个碱基对（bp）的片段，并测定了其核苷酸序列。然后将牛分枝杆菌卡介苗中mpb63的核苷酸序列与结核分枝杆菌中mpt63的核苷酸序列进行比较。结果表明，两个蛋白质基因的核苷酸序列在基因结构和上游区域相当一致，只是在下游拖尾序列中检测到一个碱基从mpt63中的C变为mpb63中的G。这表明，尽管两种微生物的特征非常相似，但牛分枝杆菌卡介苗的遗传信息与结核分枝杆菌并不完全相同。
